脱细胞异体真皮基质生物学特性的实验研究  

Study on the biological properties of allogenic acellular dermal matrix

作　　者：左金华[1] 杨佑成[1] 李武修[1] 吴淑华[1] 马云[1] 吕增华[1] 韩兆东[1] 邢月利[1] 

机构地区：[1]滨州医学院附属医院口腔颌面外科,山东滨州256603

出　　处：《临床口腔医学杂志》2004年第5期276-278,共3页Journal of Clinical Stomatology

基　　金：山东省卫生厅青年科学基金资助 (2 0 0 3CA2CFAB1 )

摘　　要：目的 :观察脱细胞真皮基质 (ADM )全层皮肤缺损创面移植后的组织学变化 ,了解ADM的生物学特点。方法 :在Wistar大鼠背部制作全层皮肤缺损创面 ,移植异体ADM ;分别于术后 1、2、3、4周大体观察ADM表面情况 ,取标本作HE染色 ,光镜观察 ,并进行血管计数检查。结果 :成纤维细胞伴随血管自创底逐渐向ADM内生长 ,于术后 2周血管浸润ADM全层。整个观察过程均无明显炎细胞浸润 ,ADM中胶原网架结构仍然保留。结论 :ADM支持成纤维细胞浸润、血管再生 ,血管化过程约在 2周内完成 ,异体移植后亦不引起免疫排斥反应。Objective: To investigate the biological properties of acellular dermal matrix (ADM) as an allograft.Method:Two layers of allogenic ADM were implanted to full-thickness skin defects made on the dorsa of rats.Respectively,the overlying layer of ADM was removed at 1 week,2 week,3 week,4 week after implanting and the deeper ADM was observed macroscopically.Then, samples were taken for histological observation on ADM,and the number of blood vessels of ADM were counted. Result: 1 week after implanting, the surface of the deeper ADM was florid , and newly developed blood vessels had penetrated two-thirds of ADM.2weeks after implanting, extensively spotty microvascular bleeding was found, and blood vessels had extended to the uppermost region of ADM.3 weeks and 4 weeks after implanting,the overlying layer of ADM gradually dried to a crust, and obvious bleeding from the deeper ADM was observed,and the number of blood vessels did not increase significantly(P>0.05).It was also found fibroblasts accompanying newly developed vessels infiltrated ADM ,but no obvious inflammatory cells infiltration at all times.Moreover, the collagen meshwork of ADM retained its original organized structure.Conclusion: ADM could support fibroblasts infiltration, neovascularization and could be completely vascularized within 2 weeks after transplanting .Also, ADM induced no immune rejection.

关 键 词：脱细胞真皮基质 成纤维细胞 血管形成 

分 类 号：Q813.1[生物学—生物工程]