深圳地区乙型肝炎病毒DNA基因分型与临床的关系  

Genotyping of HBV DNA in Shenzhen and clinical manifestations

作　　者：戴建宜[1] 石之驎 戴勇[2] 杜珙[2] 陈德珩[2] 王沙燕[2] 

机构地区：[1]暨南大学医学院附属第二医院深圳市人民医院消化内科,深圳518020 [2]临床医学研究中心

出　　处：《中华肝脏病杂志》2004年第4期199-200,共2页Chinese Journal of Hepatology

摘　　要：目的 检测血清中乙型肝炎病毒(HBV)DNA并进行基因分型。方法 采用聚合酶链反应(PCR)扩增HBV DNA后,用不同的探针,利用微板杂交法将其分为6个亚型。结果 150例HBV DNA阳性病人中,B型50例、C型36例、D型13例、F型3例和A型1例,没有发现E型。另有47例为混合型,其中以B、D型(18例)、C、D型(20例)为主,分别占混合型的38.30％与42.6％。受检病人中,B型感染者丙氨酸氨基转移酶水平明显增高;B型感染者比C型感染者抗HBe阳性率高,分别为64.0％和30.5％,而C型感染者比B型感染者乙型肝炎e抗原阳性率高,分别为47.2％和21.0％;年龄、性别与基因型关系不明显。结论 深圳地区BBV基因亚型以B型为主,C型次之,其他较少。基因型与肝炎程度有一定关系。Objective To detect HBV DNA and its genotypes. Method The 6 isoforms of HBV DNA was detected out using of different probes by Polymerase Chain Reaction and Nucleic Acid hybridization. Results Of 150 HBV DNA positive patients who lived in Shenzhen, 50 samples (33%) are type B, 36 samples (24%) are type C, 13 samples (9%) are type D, 3 samples is type F, 1 sample is type A, 48 samples (31%) are mixed type. The ALT value was significantly higher in genotype B than in genotype C. HBe positivity were higher in genotype B than genotype C. HBeAg positivity were higher in genotype C than in genotype B. There are not obvious relations between genotype and age or sex. Conclusion [n the detected samples, the major genotype of HBV DNA is type B, several are type C, D. The type E haven't been found. There are some relations between all kinds of genotypes and the severity of hepatitis B.

关 键 词：深圳地区 乙型肝炎病毒 DNA基因 基因分型 聚合酶链反应 微板杂交法 

分 类 号：R512.62[医药卫生—内科学]