丙型肝炎病毒非编码区ABC程序酶切分型研究  

Hepatitis c virus genotype research by ABC programs of 5' -NCR restriction endocuclease digestion

作　　者：邱国华[1] 杜绍财[1] 孙南雄[2] 尤鹏[1] 范晓峰[2] 张永祥[3] 魏来[1] 

机构地区：[1]北京大学人民医院肝病研究所,100044 [2]南京医科大学第一附属医院传染病研究室 [3]美国Saint Couis大学内科学胃肠与肝病科病毒性肝炎研究中心

出　　处：《中华肝脏病杂志》2004年第4期237-239,共3页Chinese Journal of Hepatology

摘　　要：+目的 为进一步了解中国是否存在HCV 3b基因及1a、2b和6a基因型感染,建立HCV 5′-端非编码区(5′-NCR)不同基因型的基因库。方法 分型方法按ABC程序进行,A应用BHH′(BsrB Ⅰ、HaeⅡ、Hinf Ⅰ)复合内切酶消化5′-NCR cDNA,可将不同基因型划分为5组:1a、1b,6a,2a、2b,3a,3b、4a。B应用BstU Ⅰ内切酶鉴别1a、1b。C应用HaeⅢ内切酶鉴别2a、2b、3b、4a及6a。电泳检测片段大小。结果 (1)1a、1b、2a、2b、3a、3b、4a、6a 8种基因型参比品的ABC分型结果表明,该8种基因型获得良好的分型效果。(2)93份HCV RNA阳性患者ABC分型结果表明,1b型感染率占66.67％,2a型18.28％,1b/2b型、3b型及2b型均为3.23％,2a/2b型和1b/2a型各为2.15％,1a型1.08％。结论 结果表明应用HCV 5′-NCRABC分型技术既保证了HCV RNA检测的灵敏度,又能完成1a～6a型中的8种基因型的鉴别。Objective In order to fully understand hepatitis c virus (HCV) genotype 3b, la, 2b and 6a infection in China, We built HCV 5'-noncoding region (5'-NCR) of different genotypes and subtypes. Methods The classification HCV into variable genotypes (subtypes) was carried on by programs A, B and C. A. Using a combination of three restriction endonuclease BHH' (BsrB Ⅰ, Hae Ⅱ, Hinf Ⅰ) digestions at the same time. The distinct genotypes were classified into 5 groups: genotype 1 (la, 1b), 6a, 2(2a, 2b), genotype 3(3a, 3b), genotpye4(4a). B. With regard to genotype l,we could distinguish subtype la from 1b using BstU I digestion. C.Using restriction endonuclease Hae Ⅲ, genotype 2a, 2b, 3b, 4a,6a are differentiated respectively. Results (l)HCV genotype la, 1b, 2a, 2b, 3a, 3b, 4a, 6a are fully discriminated by comparison with the genotypes regular samples. (2)Of the 93 patients, HCV genotype distribution in China was 66.67% for 1b, 18.28% for 2a,3.23% for lb/2b, 3b, 2b respectively. 2.15% for 2a/2b, lb/2a respectively. 1.08% for la. Conclusion This research indicated that adoption of HCV 5' -NCR ABC restriction endonuclease digestions techniques, might be sensitive and efficient to detect HCV and discriminate HCV genotype (subtypes) 1a-6a.

关 键 词：丙型肝炎病毒 非编码区 ABC程序 分型 内切酶 核糖核酸 

分 类 号：R346[医药卫生—基础医学]