限制性内切酶分析法在柯萨奇B组病毒检测及分型中的应用  

A new method to detect and subtype coxsackie B group virus-restriction enzyme analysis method

作　　者：孙非[1] 肖兰[1] 刘志屹[1] 张淑芹[1] 刘建伟[1] 李忠谊[2] 许守民[3] 

机构地区：[1]吉林大学再生医学科学研究所病毒研究室,长春130021 [2]澳大利亚联邦科学院黑山研究所 [3]东北师范大学生命科学院

出　　处：《中华检验医学杂志》2004年第1期30-33,共4页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目 (3 987172 )

摘　　要：目的　探讨限制性内切酶分析法在柯萨奇B组病毒B1～B6型检测及分型中的应用。方法　利用GCG软件对柯萨奇B1～B6型病毒cDNA全序列进行了限制性内切酶酶切位点的分析。结果　共 114种限制性内切酶在柯萨奇B1～B6型病毒cDNA中分布有不同数量的酶切位点。经过比较和分析 ,得到了它们的一个共同cDNA片段 ,在这个cDNA片段中 ,B1～B6型病毒各自具有特异性的核苷酸序列 (特异的限制性内切酶位点 ) ,对柯萨奇B组病毒 6个已知型别标准毒株进行了成功的分型和检测。结论　本方法有效地解决了在柯萨奇B组病毒检定以往方法中所存在的非特异性问题 ,可以对柯萨奇B组病毒进行准确的检定和型别鉴定。Objective To establish a new method to detect and subtype Coxsackie B group virus quickly and accurately. Method Analyze the restriction enzyme digestion websites in the complete cDNA sequences of Coxsackie B group virus with the GCG software. Result 114 kinds of restriction enzymes have different incisive websites in cDNA of Coxsackie B group virus, after comparison and analysis; we find a same cDNA sequence, in which 6 types of Coxsackie B group virus have special sequences respectively (special restriction enzyme incisive websites). Therefore, we can detect and subtype the 6 types of coxsackie B group virus successfully.Conclusion This method resolves the problem of high specificity in detecting Coxsackie B group virus by traditional ways. It can identify and subtype Coxsackie B group virus accurately.

关 键 词：限制性内切酶分析法 柯萨奇B组病毒 检测 分型 临床应用 

分 类 号：R446.5[医药卫生—诊断学]