雪旺细胞移植促进中脑损伤神经元修复的实验研究  

Study on repairing injured middle brain by Schwann cells transplantation

作　　者：林野[1] 朱庆三[1] 尹飞[1] 郭丽[2] 

机构地区：[1]吉林大学中日联谊医院,长春130031 [2]吉林大学公共卫生学院

出　　处：《中华外科杂志》2004年第3期187-189,共3页Chinese Journal of Surgery

摘　　要：目的　探讨雪旺细胞移植对损伤的大鼠中脑神经元修复的影响。方法　BrdU标记的新生大鼠雪旺细胞移植至电针损伤的大鼠中脑区域 ,不同时间处死动物 ,免疫组织化学方法观察BrdU、GAP 4 3的表达并通过图像分析系统处理实验结果。结果　雪旺细胞移植后 8个月仍可见BrdU阳性细胞 ,其数目增加 15 % ,并主要向大脑皮层迁移 ;移植后 1个月损伤的中脑神经元GAP 4 3的表达与对照组相比差异有显著意义。Objective To explore the ability of grafted Schwann cells to promote restore of injured neurons in rat. Methods Schwann cells labeled by BrdU in vitro transplanted into rat middle brain area prior to injure with electric needle stimulus. Immunohistochemistry and image analyzer were used to investigate the expression of BrdU and GAP 43 as well as quantitative analysis respectively. Results BrdU positive cells could be identified for up to 8 months and the number increased about 15%, which mainly migrated toward injured ipsilateral cortex. The GAP 43 expression reached its peak one month after transplantation and was significant compared with control group ( P <0 05). Conclusion The transplantation of Schwann cells could promote the restoration of injured neurons.

关 键 词：雪旺细胞移植 中脑损伤 神经元修复 实验研究 

分 类 号：R651.1[医药卫生—外科学]