亚砷酸钠对生长发育影响的分子遗传机制研究  

Molecular genetic mechanism of sodium arsenite on growth and development

作　　者：姚华[1] 王国荃[1] 朱滨[2] 孙悦[2] 景丰香[2] 赵建龙[2] 

机构地区：[1]新疆医科大学公共卫生学院环境卫生教研室,乌鲁木齐830054 [2]中国科学院上海冶金研究所一室

出　　处：《中华预防医学杂志》2004年第1期33-35,共3页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金资助项目 (3 99690 0 4)

摘　　要：目的　利用基因芯片技术研究亚砷酸钠对生长发育相关基因表达的影响 ,探讨砷的分子遗传学机制。方法　将正常肝细胞cDNA克隆库的基因点在芯片上 (每个克隆是什么基因尚未知 ) ,然后与暴露于不同亚砷酸钠浓度肝细胞中得到的cDNA第一链杂交 ,对表达有差异的克隆进行基因测序 ,明确是什么基因 ,最后筛选出与生长发育相关的基因。结果　两个染毒组人甲状腺激素结合蛋白基因 (p5 5 )的表达水平分别是对照组的 2 .2 1和 2 .93倍 ,说明亚砷酸钠增强了 p5 5基因的表达。两个染毒组与对照组基因表达水平相比 ,胎盘催乳素基因 (PL)分别是 0 .13和 0 .2 7,同源框基因(HOXA10 )分别是 0 .2 2和 0 .35 ,表明亚砷酸钠抑制PL基因和HOXA10基因的表达。结论　亚砷酸钠对生长发育相关基因表达有影响 ,初步揭示了砷对生长发育影响的分子遗传学机制。Objective To study the effects of sodium arsenite on gene expression related to growth and development and explored the molecular mechanism of arsenic effects using gene chips. MethodsNormal human hepatic cells were dripped on chips and then hybrided with the first strand of cDNA from hepatic cell exposd to different concentration of sodium arsenite. Gene sequence of clone differently expressed was determined and then defined which gene it was and finally those genes which associated with growth and development were identified. Results The p55 gene expression level of two experimental groups was severaly 2.21 and 2.93 times as the control group. The PL gene level of two experimental groups were 0.13 and 0.27 times as the control group, and the HOXA10 gene level was 0.22 and 0.35 times of the control group. These results indicated that sodium arsenite increase p55 gene expression, and inhibited PL and HOXA10 gene expression. Conclusions The sodium arsenite could affect the gene expression related to growth and development and it is shown that the molecular genetic mechanism of sodium arsenite is related to growth and development.

关 键 词：亚砷酸钠 生长发育 分子遗传机制 寡核苷酸序列分析 钠化合物 

分 类 号：R114[医药卫生—卫生毒理学]