植物雌激素α-玉米赤霉醇降低组织因子及核转录因子AP-1和NF-κB水平的作用  

Phytoestrogen α-Zearalanol Markedly Reduces Tissue Factor Levels and Down-regulates Transcription Factors AP-1 and NF-κB

作　　者：王雯[1] 朱广瑾[1] 祖淑玉[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所病理生理学系,邮政编码北京100005

出　　处：《微循环学杂志》2004年第2期5-7,10,共4页Chinese Journal of Microcirculation

基　　金：国家自然科学基金重点项目 ( 3 973 0 2 2 0 )

摘　　要：目的 :探讨天然植物雌激素α 玉米赤霉醇对组织因子含量的影响及对内皮细胞表达核转录因子AP 1和NF κB的作用。方法 :建立大鼠去卵巢模型 ,进行人脐静脉内皮细胞体外培养。用放射免疫法测定大鼠血浆雌二醇水平 ;酶联免疫吸附法测定血浆及内皮细胞组织因子水平。用WesternBlot法测定核转录因子AP 1和NF κB的含量。结果 :去卵巢后 ,大鼠血浆组织因子水平升高 ,补充α 玉米赤霉醇后可使升高的血浆组织因子接近正常 ;α 玉米赤霉醇亦可降低内皮细胞组织因子含量 ,且可下调内皮细胞核转录因子AP 1和NF κB的水平 ,以上作用均与 17β 雌二醇类似。结论 :α 玉米赤霉醇可能具有抗血栓形成 。Objective: To investigate the effects of phytoestrogen α-zearalanol on tissue factor (TF) and transcription factors AP-1 and NF-κB in plasma of ovariectomized (OVX) rats and human umbilical vein endothelial cells (HUVECs), respectively.Method: The concentrations of rats plasma estradiol were measured by radioimmunoassay. The TF levels of rats plasma and HUVECs were assayed by enzyme-linked immunosorbent assay (ELISA). Nuclear proteins of HUVECs were extracted to assay transcription factors AP-1 and NF-κB by Western blot.Results: That α-zearalanol have similar effects with 17β-estradiol to either decrease TF levels in OVX rats and HUVECs or down-regulate AP-1 and NF-κB levels in HUVECs.Conclusion: These results suggest that α-zearalanol might inhibit expression of TF, which maybe contribute to anti-thrombosis and protection of cardiovascular system.

关 键 词：植物 雌激素 Α-玉米赤霉醇 组织因子 核转录因子 AP-1 NF-ΚB 心血管系统 

分 类 号：R318[医药卫生—生物医学工程]