高浓度游离脂肪酸对胰岛细胞凋亡及相关基因表达的影响  

Effects of High Level of Free Fatty Acid on the Apoptosis and Expression of Apoptosis-related Genes of Islet Cells

作　　者：何庆[1] 王保平[2] 刘铭[3] 苏京[2] 王晶[2] 陈克勤[2] 尹潍[1] 

机构地区：[1]天津医科大学总医院内分泌科,300052 [2]丹麦诺和诺德中国研究发展中心 [3]天津医科大学总医院老年病研究室,300052

出　　处：《天津医药》2004年第5期293-295,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金资助课题(项目编号:983702411)

摘　　要：目的 :观察高浓度游离脂肪酸体外对胰岛细胞凋亡和凋亡相关基因表达的影响。方法 :应用TUNEL法检测高浓度游离脂肪酸培养后大鼠胰岛细胞和小鼠 βTc3细胞凋亡百分率 ,应用定量RT -PCR(QRT -PCR)检测培养后bcl-2、bax、c-myc、p53和fasmRNA的表达。结果 :高浓度游离脂肪酸使原代培养的大鼠胰岛细胞和βTc3的凋亡细胞比例明显增加。胰岛细胞凋亡过程中 ,诱导凋亡基因bax、c -myc、fas的mRNA表达水平明显升高 ,抵抗凋亡基因bcl-2mRNA表达水平明显下降 ,p53mRNA表达水平无明显改变。结论 :游离脂肪酸可能通过诱导胰岛细胞凋亡导致或加重糖尿病 。Objective:To investigate effects of high level of free fatty acid on the apoptosis and expression of apoptosis-related genes of islet cells.Methods:Apoptotic cells were identified by TUNEL method.The mRNA levels of bcl-2、bax、c-myc、p53and fas were evaluated by the methods of quantitative RT-PCR.Results:High level of the free fatty acids increased the proportion of the apoptotic cell of primary rat islet cell andβTc3cell line.In the process of the apoptosis,the mRNA level of the bax c-myc,and fas increased sigˉnificantly,bcl-2mRNA's expression decreased,and p53's mRNA expression was not changed significantˉly.Conclusion:High level of free fatty acids may initiate and exacerbate the diabetes through inducing the apoptosis of islet cells,the change of bcl-2/bax mRNA expression ratio may play an important role in the apoptosis.

关 键 词：游离脂肪酸 胰岛 细胞凋亡 基因表达 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]