高度糖基化终产物对大鼠主动脉平滑肌细胞G-蛋白亚基基因表达的影响  

Regulation of G-protein subunit mRNA expression in rat aortic smooth muscle cell by advanced glycosylation end products

作　　者：胡向阳[1] 黄晓明[1] 刘乃丰[2] 金辉[2] 

机构地区：[1]东南大学医学院现代医学实验中心,江苏南京210009 [2]东南大学附属中大医院心脏科,江苏南京210009

出　　处：《东南大学学报（医学版）》2004年第3期145-147,共3页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目 (39470 31 4 )

摘　　要：目的 :研究高度糖基化终产物 (AGEs)对G 蛋白亚基基因表达的影响 ,了解G 蛋白在AGEs介导的胞内信号转导途径中的作用。方法 :利用半定量RT PCR技术分析大鼠主动脉平滑肌细胞受AGEs作用后G 蛋白亚基Goα、GsαmRNA的改变。结果 :AGEs在大鼠主动脉平滑肌细胞中上调Gsα的表达 ,但却使GoαmRNA水平显著下降。结论 :GObjective To study the effects of advanced glycosylation end products(AGEs) on the expression of G?protein subunit mRNA,and to evaluate the significance of G?protein in AGEs-mediated signal transduction pathways.Methods Alternation of G?protein subunit mRNA level in cultured rat aortic smooth muscle cell(ASMC) after exposure to AGEs was determined by a semi-quantitative reverse-transcription polymerase chain reaction(RT?PCR).Results The expression of Gsα subunit mRNA was up-regulated at least two folds than in the controls by AGEs in ASMC,whereas the levels of Goαsubunit mRNA declined significantly.Conclusion Some G?protein subunits may be involved in the AGEs-mediated cellular signal transduction pathways and pathological processes.

关 键 词：高度糖基化终产物 大鼠 主动脉平滑肌细胞 G-蛋白亚基 基因表达 细胞培养 信号转导途径 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]