低氧促进大鼠心脏成纤维细胞DNA合成及Ⅰ、Ⅲ胶原mRNA表达  

HYPOXIA PROMOTE THE DNA SYNTHESIS AND THE EXPRESSION OF COLLAGEN TYPE I AND III mRNA IN CULTURED ADULT RAT CARDIAC FIBROBLASTS

作　　者：严俊[1] 高钰琪[1] 谢增柱[1] 

机构地区：[1]第三军医大学高原军事医学系病理生理学与高原生理学教研室,重庆400038

出　　处：《中国应用生理学杂志》2004年第2期125-128,共4页Chinese Journal of Applied Physiology

摘　　要：目的 :研究低氧 (2 %氧 )对成年Wistar大鼠心脏成纤维细胞DNA合成及Ⅰ、Ⅲ型胶原前α肽链表达的影响。方法 :分离培养成年Wistar大鼠心脏成纤维细胞 ,采用液体闪烁计数方法检测心脏成纤维细胞的DNA合成速率 ,采用原位杂交技术检测Ⅰ、Ⅲ型胶原前α肽链mRNA的表达。结果 :成年Wistar大鼠心脏成纤维细胞在低氧第 6h、12h时3 H TdR掺入量较常氧组显著增加 ,分别增加 34% (P <0 .0 5 )和 36 % (P <0 .0 1) ;低氧第 4h、8h、12hI型胶原前α肽链mRNA表达显著高于常氧培养的细胞 ;低氧第 2h ,Ⅲ型胶原前α肽链mRNA表达显著高于常氧培养的细胞。结论 :低氧能够直接促进体外培养的成年Wistar大鼠心脏成纤维细胞DNA合成和Ⅰ、Ⅲ型胶原前α肽链表达 ,提示低氧对心脏成纤维细胞生长和胶原表达的直接调节可能是低氧性心肌纤维化的重要机制。Aim: To observe the effects of hypoxia on DNA synthesis and the expression of collagen type Ⅰ and Ⅲ mRNA in cultured adult rat cardiac fibroblasts. Methods: Cardiac fibroblasts(CFs) were isolated from adult Wistar rat ventricule and cultured in vitro either in normoxic or hypoxic condition. Studies were conducted with the second passage of CFs. The changes of DNA synthesis was determined by measuring the incorporation of 3H-TdR into DNA and the changes of expression of pro-α1(Ⅰ) collagen, pro-α1(Ⅲ) mRNA were measured by in situ hybridization respectively. Results: The 3H-TdR incorporation of CFs was increased by 34%(P<0.05) and 36%(P<0.01) after 6 h, 12 h hypoxia(2% O 2) exposure respectively. The level of pro-α1(Ⅰ) collagen mRNA expression was significantly elevated in the cells under hypoxia for 4 h, 8 h, and 12 h.The expression of pro-α1(Ⅲ) mRNA increased when cells were cultured under hypoixa for 2 h. Conclusion: These results suggest that hypoxia alone can upregulate DNA synthesis and expression of collagen type Ⅰ and Ⅲ mRNA in adult rat cardiac fibroblsts. It may be one of the important mechanisms by which hypoxic myocardial fibrosis occur.

关 键 词：低氧 大鼠 心脏成纤维细胞 DNA 合成 胶原 表达 

分 类 号：R331[医药卫生—人体生理学]