褪黑素抑制小鼠卵母细胞的体外成熟  

THE SUPRESSION OF MELATONIN ON MOUSE OOCYTE IN VITRO MATURATION OF MOUSE

作　　者：汪建红[1] 卜淑敏[1] 夏国良[1] 王松波[1] 陶勇[1] 雷蕾[1] 谢辉蓉[1] 周波[1] 

机构地区：[1]中国农业大学生物学院

出　　处：《中国应用生理学杂志》2004年第2期197-200,共4页Chinese Journal of Applied Physiology

基　　金：国家杰出青年基金资助项目 ( 3 0 0 2 5 0 3 2 )

摘　　要：目的 :探讨褪黑素 (MT)对小鼠卵母细胞的体外成熟的影响。方法 :通过卵母细胞自发、次黄嘌呤 (HX)阻滞和激素诱导成熟三种体外培养模型研究了褪黑素 (MT)对小鼠卵母细胞体外成熟的影响。结果 :① 0 .1g/L、0 .0 2g/L、0 .0 0 4 g/L及 0 .0 0 0 8g/L浓度的MT均能显著抑制小鼠卵丘卵母细胞复合体 (CEOs)自发成熟过程中第一极体 (PB1)的释放 (P <0 .0 1) ;②动力曲线分析表明 ,MT对自发成熟的CEOs的GVBD和PB1有显著的推后作用 ,与对照组相比 ,处理组的GVBD和PB1分别被推后 8～ 10h和 3～ 4h ;③ 0 .1g/L和 0 .0 2g/L两有效浓度的MT还能显著抑制促性腺激素 (FSH)诱导的HX阻滞的CEOsGVBD的发生 (P <0 .0 5 ) ,对PB1的排出虽有一定的抑制作用 ,但没有统计学意义 ;④MT和次黄嘌呤 (HX)对CEOs的自发成熟有协同抑制作用 (P <0 .0 1) ,但在裸卵 (DO)自发成熟的阻滞中没有协同效应。结论 :MT是调节哺乳动物卵母细胞成熟的重要激素之一 ,其作用机制可能是通过卵丘细胞实现的。Aim: To study whether metlatonin has effect on oocyte maturation of mouse in vitro. Methods: Mouse oocytes were cultured in maturation medium, HX-medium, or HX-medium supplemented with FSH, and the effects of MT on meiotic maturation of mouse oocyte were examined. Results: ① MT at all doses of 0.1 g/L、0.02 g/L、0.4 g/L or 0.8 g/L inhibited the formation of PB1 in CEO cultured in maturation medium and had no effect on GVBD. ② MT could delay GVBD and the extrusion of PB1 in CEOs of mouse oocytes by dynamic curves. In contrast to the control, GVBD and PB1 extrusion of oocytes in the treated groups had been delayed by 8～10 hours and 3～4 hours respectively. ③ MT inhibited the effect of FSH on resumption of meiosis, but no effect on the formation of PB1. ④ MT and HX had cooperation effects on spontaneous oocyte maturation in CEO , but not in DO. Conclusion: MT is able to affect mouse oocyte maturation and the regulation mechanisms may be related to cumulus cells.

关 键 词：褪黑素 小鼠 卵母细胞 体外成熟 第一极体 哺乳动物 

分 类 号：Q492.5[生物学—生理学] R339.22[医药卫生—人体生理学]