沙眼衣原体酶免疫法和细胞培养法的比较  

Detection of Urogenital Chlamydia Trachomatis with Enzyme Immunoassay and Cell Culture

作　　者：张津萍[1] 钟铭英[1] 张树文[1] 王千秋[1] 

机构地区：[1]中国医学科学院中国协和医科大学皮肤病研究所,江苏南京210042

出　　处：《中国皮肤性病学杂志》2004年第5期288-289,共2页The Chinese Journal of Dermatovenereology

摘　　要：目的　评价一种改进的沙眼衣原体酶免疫法 (ELISA)诊断沙眼衣原体感染的临床应用价值。方法　 2 0 0例性病门诊有泌尿生殖道症状的患者采用ELISA和细胞培养法检测泌尿生殖道标本中的沙眼衣原体 ,两种试验结果不符合者采用PCR检测。结果　 2 0 0例受检者中 ,ELISA阳性 19例 ,细胞培养阳性 2 0例。与“扩大金标准”比较 ,ELISA法和细胞培养的敏感性分别为 82 .68%和 68.96% ,特异性均为 10 0 % ,阳性预期值均为 10 0 %。结论　ELISA法检测泌尿生殖道沙眼衣原体感染 ,其敏感性和特异性高 ,且方便、快速 ,尤其适合大批量样本的检测 ,值得在临床应用。Objective To Evaluate the performance of a c om mercial available ELISA kit for the diagnosis of Chlamydia trachomatis infection s.Methods Genital specimens were tested with ELISA and cell culture in 2000 male or female patients with genital symptoms.The discrepant re sults were resolved by application of a PCR assay.Results Among 200 patients,C.trachomatis was detected in 19 by ELISA and 20 by cell culture.In comparison with expanded gold standerd,the sensitivities,specificities,positive predictive values of ELISA and cell cultur e were 82.61% and 86.96%,100% and 100% and 100%,respectively.Conclusion It is indicated that ELISA is simple,rapid,hig h sensitive and specific for the diagnosis of genital C.trachomatis infection.It is suitable for the testing of large batches of clinical specimens and worthwhi le for clinical application.

关 键 词：沙眼衣原体 细胞培养 酶联免疫吸附试验 检测 泌尿生殖道感染 

分 类 号：R374.1[医药卫生—病原生物学]