流式细胞仪测定单克隆抗体识别福氏2a痢疾杆菌及其突变株表面抗原分子表达的分析  

Preliminary Analysis of Surface Antigen Expression on Shigella flexneri 2a using Flow Cytometry

作　　者：李红[1] 彭红[1] 高杰英[1] 张双喜[2] 

机构地区：[1]军事医学科学院微生物和流行病研究所,北京100850 [2]军事医学科学院基础医学研究所

出　　处：《单克隆抗体通讯》1992年第3期13-17,共5页

摘　　要：本文报告应用流式细胞仪测定4株单克隆抗体(McAbs)识别的抗原分子在福氏2a痢疾杆菌及其突变株表面表达情况,结果表明4株McAbs所识别的抗原分子在福氏2a痢疾杆菌有毒株表面的表达高于其无毒和突变株;同一菌株中不同抗体表面“0”糖链抗原分子的表达具有明显的不均一性,这进一步说明痢疾杆菌的抗原结构非常复杂。In the present experiment, flow cytometry was employed for quantitative determination of expression of antigenic molecules on the surface of Shigella flexnerr 2a and its mutants using 4 monoclonal antibodies (McAbs) against the O- polysaccharid-es of Shigella flexneri 2a. A difference was observed in the expression of the O-polysaccharide molecules, between the strains. The findings suggested that expression of antigenic molecules in virulent strain was higher than that of avirulent strain and its mutant. However, the strains under study were highly heterogeneous in the expression of O-polysaccharide antigenic molecules.

关 键 词：流式细胞仪 抗原分子 单克隆抗体 

分 类 号：R392.11[医药卫生—免疫学]