绵羊粪便中毛圆线虫卵培养和感染性幼虫分离法的比较  

A COMPARISON OF EGG CULTIVATION AND INFECTIVE LARVAL ISOLATION OF TRICHOSTRONGYLIDAE FROM SHEEP FAECES

作　　者：王治才[1] 徐志光[2] 李建军[1] 玛利亚木[1] 王琳[1] 

机构地区：[1]新疆畜牧科学院兽医研究所,乌鲁木齐830000 [2]新疆农业大学动物医学院,乌鲁木齐830063

出　　处：《中国兽医寄生虫病》2004年第2期4-6,共3页Chinese Journal of Veterinary Parasitology

摘　　要：目的　为比较毛圆线虫卵培养和感染性幼虫分离的几种方法。方法　采用培养中添加锯末,蛭石培养羊粪及分离幼虫用纱布袋浸泡、贝尔曼分虫法、广口罐分离法。结果　在绵羊粪便毛圆线虫卵的培养中,用锯末作为添加物较蛭石作添加物,幼虫的孵化率提高1.8倍,两者差异显著(P≤0.05);用纱布袋浸泡法进行粪便培养物中感染性幼虫的分离,较贝尔曼分虫法提高3.7倍;较广口罐分离法提高2.6倍。结论　以上方法分离的感染性幼虫再经蔗糖垫层离心提取后,可得到纯净、高密度的活性虫体。The larval recovery rate increases 1.8 times with the additive of sawdust than with vermiculite in cultivation of eggs of Trichostrongylidae from sheep faeces, and the difference is significant ( P ≤0.05). The isolation rate of infective larvae with the method of gauze bag dip-in is 3.7 times higher than Baermann method, and 2.6 times higher with method of wide-mouthed jars. Clean and high density infective larvae could be obtained after centrifugation by sucrose cushion with the larvae isolated with above methods.

关 键 词：绵羊 粪便 毛圆线虫 卵培养 感染性 幼虫分离法 

分 类 号：S858.26[农业科学—临床兽医学]