当归HPLC指纹图谱研究  

Studies on the Fingerprints of Radix Angelicae Sinensis

作　　者：董自波 洪敏[2] 樊宏伟[2] 闵春艳[2] 朱荃[2] 

机构地区：[1]山东鲁南制药有限公司,山东临沂276003 [2]南京中医药大学规范化中药药理实验室,江苏南京210029

出　　处：《中国实验方剂学杂志》2004年第3期1-3,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家十五攻关项目 (2 0 0 1BA70 1A2 9)

摘　　要：目的 :应用HPLC法进行当归指纹图谱研究。方法 :Zorbax 30 0SBC1 8分析柱 ,CH3CN H2 O H3PO4 梯度洗脱 ,流速 1ml min ,检测波长 2 80nm。结果和结论 :建立了当归的指纹图谱检测标准 ,共有指纹峰 16个 ,鉴定其中三个为阿魏酸、洋川芎内酯H、洋川芎内酯I。实验方法简便、准确、重复性好。Objective:To study the fingerprints of Radix Angelicae Sinensis.Method:Zorbax 300SB C_ 18 column was used,with a mixture liquid of CH_3CN-H_2O-H_3PO_4 mobile phase in a gradient mode.The wavelength of measurement was 280nm.Result and conclusion:Sixteen peaks has been gotten from the Radix Angelicae Sinensis as its fingerprints.Three of them were identified as Ferulic acid,Senkyunolide H and Senkyunolide respectively.The method is simple and reliable.

关 键 词：当归 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]