应用聚羟基丁酸/聚羟基戊酸共聚物构建组织工程心脏瓣膜的实验研究  

In vitro experience in tissue engineering heart valve with poly-hydroxybutyrate-co-hydroxyvalerate

作　　者：周栋[1] 汪钢[1] 赵东锷[2] 刘维永[1] 金锋[1] 

机构地区：[1]第四军医大学西京医院心血管外科研究所 [2]武警总医院胸心外科,北京100039

出　　处：《中国胸心血管外科临床杂志》2004年第2期116-119,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金资助项目 ( 3 9970 73 3 )~~

摘　　要：目的　探讨应用聚羟基丁酸 /聚羟基戊酸共聚物 ( poly- hydroxybutyrate- co- hydroxyva lerate,PHBV)构建组织工程心脏瓣膜的可行性。　方法　应用扫描电子显微镜观察 PHBV的结构特点 ,将 PHBV行兔皮下包埋 ,分别于包埋后 2周、4周、6周、8周和 10周观察 PHBV的生物相容性和降解率。体外培养犬主动脉瓣间质细胞 ,观察其生长曲线 ,平滑肌 α肌动蛋白表达和透射电子显微镜下特点。将体外培养的犬主动脉瓣间质细胞种植于 PHBV上 ,观察其生长情况并测定细胞合成前列环素的功能。　结果　扫描电子显微镜下 PHBV呈网孔状泡沫结构 ,孔径 130μm。皮下包埋显示 PHBV生物相容性好 ,体内降解时间约 10周。犬主动脉瓣间质细胞平滑肌 α肌动蛋白免疫组化染色部分细胞呈阳性表达 ,细胞内有大量粗面内质网 ,生长曲线良好。种植实验显示细胞在 PHBV上生长良好 ,并有合成、分泌前列环素的功能。　结论　以 PHBV为支架构建组织工程心脏瓣膜 ,种子细胞不仅可以在 PHBV上生长 ,而且可以合成血管活性物质 ,初步提示应用Objective To evaluate the feasibility to fabricate tissue engineered heart valve leaflet with poly hydroxybutyrate co hydroxyvalerate (PHBV) scaffold. Methods Constructive characteristics of PHBV were observed by scanning electron microscope. PHBV was implanted subcutaneously in rabbit and extirpated at 2,4,6,8, and 10 weeks in order to study its biocompatibility and biodegradability. Cell growth curve, smooth muscle α actin and ultra microscopic characteristics were observed in canine aortic valve interstitial cells. The cells were seeded on the PHBV and their function of prostaglandin I 2 (PGI 2) synthesis was determined. Results Porous diameter of PHBV was 130μm, PHBV had good biocompatibility and completely degraded at the end of the 10th week. Smooth muscle α actin were expressed in canine aortic valve interstitial cells and grew well. Cells implanted on PHBV grew well, PGI 2 were synthesized and secreted( P <0.05). Conclusion It is feasible to fabricate a tissue engineering heart valve leaflet in vitro with PHBV scaffold.

关 键 词：聚羟基丁酸 聚羟基戊酸共聚物 组织工程 心脏瓣膜 PHBV 主动脉瓣 

分 类 号：R654.2[医药卫生—外科学]