11,12-环氧二十碳三烯酸对幼兔心脏再灌注性心律失常的影响  

Effects of 11,12-epoxyeicosatrienoic acid on reperfusion arrhythmias in the immature rabbit hearts

作　　者：周明阳[1] 吴清玉[1] 钟星 龙村[3] 温复兴[3] 覃筱燕[4] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院心血管外科,北京100037 [2]中国医学科学院肿瘤研究所,北京100045 [3]阜外心血管病医院麻醉与体外循环研究室,北京100037 [4]中央民族大学生物化学系,北京100089

出　　处：《中国胸心血管外科临床杂志》2004年第2期124-126,共3页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

摘　　要：目的　为提高未成熟心肌的保护作用 ,观察 11,12 -环氧二十碳三烯酸 ( 11,12 - EET)对幼兔冷停搏长时间保存的心脏再灌注性心律失常的影响。　方法　 16只幼兔按单纯随机原则分成 2组 ,每组 8只。对照组 :采用 St.Thomas 心脏停搏液保护心肌 ;实验组 :采用 St.Thomas +11,12 - EET心脏停搏液保护心肌。采用 L angendorff灌注装置 ,测定灌注心脏停搏液后的电机械活动停止时间以及保存 16小时 ( 4°C)后复灌 30分钟 ( 37°C)的心率 ( HR)、冠状动脉流量 ( CF)、心肌含水量 ( MWC)、冠状动脉漏出液中肌酸激酶 ( CK)和乳酸脱氢酶 ( L DH)、心肌钙离子含量 ,观察心律失常发生情况。　结果　实验组灌注心脏停搏液后的电机械活动停止时间明显缩短 ;HR恢复、CF、MWC、CK、L DH、心肌钙离子含量和心律失常情况均优于对照组。　结论　 St.Thom as 液中加入 11,12 -Objective To improve the myocardial protection result, observe the effects of 11,12 epoxyeicosatrienoic acid (11,12 EET) on reperfusion arrhythmias in the isolated perfused immature rabbit hearts, which underwent long term preservation. Methods Sixteen isolated rabbit hearts were randomly assigned to two groups, 8 rabbits each group. Control group: treated with St.Thomas Ⅱ solution, experimental group: treated with St.Thomas Ⅱ solution plus 11,12 EET. By means of the Langendorff technique, these isolated rabbit hearts were arrested and stored for 16 hours with 4℃ hypothermia, and underwent 30 minutes of reperfusion(37℃). The mean times until the cessation of both electrical and mechanical activity were measured after infusion of cardioplegia. The heart rate (HR), coronary flow (CF), myocardial water content (MWC), value of creatine kinase (CK) and lactic dehydrogenase (LDH), myocardial calcium content and the arrhythmias score (AS) during the period and at the endpoint of the reperfusion were observed. Results The times until electrical and mechanical activity arrest in the experimental group were significantly shorter than those in control group ; HR, CF, MWC, CK, LDH, myocardial calcium content and AS were significantly better than those in control group. Conclusions These data suggest that 11,12 EET added to the cardioplegic solution of St.Thomas Ⅱ has lower incidence rate of reperfusion arrhythmias.

关 键 词：环氧二十碳三烯酸 兔 心脏再灌注 心律失常 未成熟心肌 心肌细胞 

分 类 号：R541.7[医药卫生—心血管疾病]