雪花莲凝集素基因(GNA)植物表达载体的构建及其对烟草的遗传转化  

Construction of plant expression vector containing GNA gene and its transformation into tobacco

作　　者：柴红梅[1] 姚春馨[1] 张绍松[1] 黄兴奇[1] 

机构地区：[1]云南省农业科学院生物技术研究所,云南昆明650223

出　　处：《西南农业学报》2004年第B05期66-68,共3页Southwest China Journal of Agricultural Sciences

基　　金：云南省应用基础研究(97C001G)

摘　　要：将雪花莲凝集素基因(GNA)取代GUS基因正向插入到Ti质粒载体pBI121的35S启动子之下,构建成植物表达载体pBI121/GNA,并将其导入农杆菌LBA4404中。通过该农杆菌介导转化烟草红花大金元,得到卡那霉素抗性植株159株,PCR检测表明其中102株为阳性。对8株PCR阳性植株进行了抗蚜虫生物活性检测,抑制蚜虫密度为25％-90％。A plant expression vector pBI121/CNA was construction by replacing CUS gene in pBI121 with Galanthus ni-valis Agglutinin (CNA) .CNA gene was transferred into tobdcco mediated by Agrobacterium tumefaciens and Kanamycin-resistance transformants were got. PCR analysis indicated that CNA gene has been integrated into the tobacco genome. The results from insect bioassay with peach aphid (Mizus persicae) showed that the transgenic plants were average aphid-resistant evidenced by 25%-90% reduction in insect population density.

关 键 词：雪花莲凝集素 基因 GNA 植物 表达载体 蚜虫 生物活性 烟草 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] Q943.2[农业科学—植物保护]