地甘口服液对环磷酰胺所致小鼠脾组织凋亡及相关基因mRNA表达的影响  

Effect of Digan Oral Liquid on Expression of Apoptosis in the Spleen Tissue and Apoptosis-related Genes mRNA with CTX Lesion Mice

作　　者：何东初[1] 王晓红[1] 吴江平[1] 陈如泉[2] 

机构地区：[1]广州军区武汉总医院,湖北武汉430071 [2]湖北中医学院附院,湖北武汉430000

出　　处：《中国中医药信息杂志》2004年第5期395-396,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：湖北省自然科学基金资助(97J080)

摘　　要：目的 探讨地甘口服液对环磷酰胺损伤所致小鼠脾组织凋亡及凋亡相关基因bcl-2和bax mRNA表达的作用。方法 建立环磷酰胺损伤的小鼠模型(小鼠用环磷酰胺100mg/kg,腹腔注射1次/d,连续3d,造成模型),喂饲100%地甘口服液每次0.2mL/10g小鼠,每日2次。第10天用原位末端标记的TUNEL技术检测脾组织细胞凋亡和原位杂交法检测凋亡相关基因bcl-2和bax mRNA的表达。结果 地甘口服液组与对照组比较,脾脏的细胞凋亡率和bax mRNA表达显著减少(P<0.01),bcl-2 mRNA的表达显著增强(P<0.01)。结论 地甘口服液可能通过促进bcl-2和降低bax mRNA的表达,促进造血细胞的增生和提高机体免疫力。Objective To study the effect of Digan oral liquid (DGOL) on expression of apoptosis in the spleen tissue and some apoptosis-related genes mRNA with CTX lesion mice. Methods After the model of chemical lesion given CTX of 100mg/kg mouse by intraperitoneal injection was established, the animals were fed with 0.2mL/10g of 100% DGOL. Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) insitu hybridiation. Results In the DGOL group, the apoptosic cells ration was significantly decreased (P<0.01) and bcl-2 mRNA expression was significantly increased (P<0.01) in spleen tissue than control group. Conclusion DGOL can restrain the apoptosis of spleen tissue, due to the enhanced bcl-2 and the decreased bax in mRNA expression with radiation lesion, thus prevent the blood cells from injuring and premote the immunity.

关 键 词：地甘口服液 环磷酰胺 小鼠 脾组织凋亡 基因表达 MRNA表达 化学损伤 

分 类 号：R269.576[医药卫生—中西医结合] R286[医药卫生—中医外科学]