核桃离体繁殖技术  

Micropropagation Techniques for Three Walnut Cultivars

作　　者：吕守芳[1] 闫爱玲[2] 吴燕民[3] 奚声珂[1] 

机构地区：[1]中国林科院林业所,北京100091 [2]北京市农林科学院林果所,北京100093 [3]中国农科院生物技术中心,北京100081

出　　处：《经济林研究》2004年第1期12-14,共3页Non-wood Forest Research

基　　金：国家林业局"核桃引种研究"课题的部分内容 ;北京市青年科技骨干培养基金资助项目"核桃品种离体繁殖技术的研究"。

摘　　要：对核桃JuglansregiaL.辽核1号、辽核4号、元丰3个品种的茎段进行诱导芽分化、生长和生根成苗试验。筛选出茎段离体初代培养基为DKW+BA1.0mg/L;继代培养和壮苗培养基为DKW+BA1.0mg/L+IBA0.01mg/L,诱导生根培养基为1/2DKW+IBA5mg/L,加活性碳1mg/L和间苯三酚1mg/L,生根率达95%以上,重复性稳定。Three walnut cultivars of 'Liaohe 1', 'Liaohe 4', and 'Yuanfeng' were propagated in vitro. Stem fragments were used for bud differentiation, bud growth, and plantlet rooting. Explantlets were initiated on basal DKW with hormone (BA 1.0 mg/L). The new shoots that had emerged from the primary buds were successively transferred to DKW with hormones (BA 1.0 mg/L and IBA 0.01 mg/L). The rooting medium was 1/2 DKW with hormone (IBA 5 mg/L) plus active carbon at 1.0 mg/L and phloroglucinol at 1.0 mg/L, and the rooting percentage exceeded 95 percent with stable repetitiveness.

关 键 词：核桃 离体繁殖技术 生根诱导 品种类型 

分 类 号：S664.1[农业科学—果树学]