酶联免疫分析结合生物素-亲合素放大体系测定血清中的雌三醇  

Determination of E3 in Serum by EIA with Biotin-Avidin Amplification System

作　　者：赵金富[1] 王永成[1] 米健秋[1] 常文保[1] 

机构地区：[1]北京大学化学与分子工程学院,北京100871

出　　处：《高等学校化学学报》2004年第6期1019-1022,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 0 75 0 0 1)资助

摘　　要：通过简单的全合成获得了共价结合的雌三醇 -生物素复合物 ( E3 -Biotin) ,结合辣根过氧化物酶标记的亲合素 ( HRP-Avidin)作为免疫分析探针 ,建立了灵敏的酶联吸附免疫分析新方法以测定血清中的雌三醇 .在稀释酶的缓冲溶液中加入小牛血清可有效地抑制非特异性吸附 .实验结果表明 ,此方法具有很高的灵敏度和抗非特异性吸附干扰的能力 ,同时具有很好的稳定性和重现性 .标准曲线线性范围为 0 .1～ 1 0 ng/ m L,检出限为 0 .0 6ng/ m L.The complex of Estriol and Biotin(E3-Biotin) was prepared by a simple procedure. E3-Biotin and horseradish peroxidase-labelled avidin(HRP-Avidin) were regarded as a probe in enzyme immunoassay(EIA). A sensitive EIA was developed and applied to determining E3 in serum. The unspecific adsorption of HRP-Avidin was inhibited by adding the calf serum into enzyme buffer. The experimental results indicated that the higher sensitivity and better stability and repeatability were achieved without any influence from the unspecific adsorption. The calibration curve covered a range from 0.1 to 10 ng/mL,and the limit of detection was 0.06 ng/mL(n=10,3σ/N). [WT5HZ]

关 键 词：雌三醇 酶免疫分析 生物素 亲合素 

分 类 号：O657[理学—分析化学]