维生素C对Hela细胞系增殖及细胞周期的影响  

Effect of Vitamin C on Cell Proliferation and Cell Cyclekinetics of Hela Cell Line in Vitro

作　　者：李能[1] 周波[1] 陈忠东[1] 

机构地区：[1]南华大学第一附属医院,湖南衡阳421001

出　　处：《南华大学学报（医学版）》2004年第2期161-163,共3页Journal of Nanhua University(Medical Edition)

摘　　要：目的　探讨维生素C对Hela细胞系增殖及细胞周期的影响。方法　采用MTT测定 ,观察不同浓度维生素C( 1 μmol/L～ 35 0 0 μmol/L)对Hela细胞系增殖的抑制作用 ;运用流式细胞仪检测用药后细胞周期分布及增殖指数的变化。结果　各个浓度的维生素C对Hela细胞系增殖均有抑制作用 ,并呈剂量依赖性 ,35 0 0 μmol/L时 ,抑制率为 66.1 5 %。维生素C能使Hela细胞系阻滞于G0 /G1 期 ,抑制DNA的合成 ,使细胞增殖指数明显下降。且此作用随用药浓度的增加而增强。结论　维生素C在体外对Hela细胞系有明显的抑制作用 ,使细胞阻滞于G0 /G1 期。Objective To investigate the effects of vitamin C on cell proliferation and cell cycle kinetics of Hela cell line in vitro. Methods The antiproliferation effects of different concentrations of vitamin C(1～3 500 μmol/L)on Hela cell line were detected by MTT assay.Flow cytometry analysis technique were used to determine the cell cycle distribution and cell proliferation index after treatment with vitamin C.Results Vitamin C inhibited the proliferation of Hela cell line on a dose-depending manner.The inhibition reached a plateau at 3 500 μmol/L(66.15% inhibition) by vitamin C. The cell cycle progression was blocked in G 0/G 1 phase and reduced proliferation.Conclusion In vitro,vitamin C significantly inhibits the growth of Hela cell line and arrests the cell cycle progression in G 0/G 1 phase.

关 键 词：维生素C 流式细胞术 HELA细胞系 

分 类 号：R977.23[医药卫生—药品]