流动注射化学发光测定硫酸小诺霉素及其机理探讨  

Flow Injection Chemiluminescence Analysis of Micronomicin

作　　者：罗万芬[1] 章竹君[2] 王周平[2] 付志锋[2] 方卢秋[3] 

机构地区：[1]西南农业大学荣昌校区基础部,重庆402460 [2]西南师范大学分析科学研究所,重庆400715 [3]涪陵师范学院化学系,重庆408003

出　　处：《分析科学学报》2004年第3期248-250,共3页Journal of Analytical Science

基　　金：国家自然科学基金(No.20175039)

摘　　要：在碱性介质中,硫酸小诺霉素能被N 溴代琥珀酰亚胺(NBS)氧化,在荧光素的增敏作用下,产生化学发光。基于此,建立了测定痕量硫酸小诺霉素的流动注射化学发光分析法。硫酸小诺霉素在0.5～100μg·mL-1的浓度范围内与化学发光强度呈良好的线性关系,检出限(3σ)为0.08μg·mL-1,对5μg·mL-1的硫酸小诺霉素连续测定11次,相对标准偏差为1.8%。将本方法用于制剂中硫酸小诺霉素的分析,结果满意。A new method for the determination of micronomicin with flow injection chemiluminescence was described. It was based on the chemiluminescence during the oxidation of micronomicin by N-bromosuccinimide (NBS) in alkaline medium in the presence of fluorescein as energy transfer. This method was sensitive, simple, rapid and reproducible. The linear range for micronomicin was 0.5～100 μg·mL^(-1 )with the relative standard deviation of 1.8% (c=5.0 μg·mL^(-1), n=11). The detection limit (3σ) was (0.08) μg·mL^(-1). The recommended method has been successfully used for the analysis of commercial formulation with satisfactory results.

关 键 词：流动注射分析 化学发光 硫酸小诺霉素 N-溴代琥珀酰亚胺 荧光素 

分 类 号：O657.39[理学—分析化学] R978.1[理学—化学]