提高维甲酸毛细管电泳分析灵敏度的微柱固相萃取法研究  

Investigation on Improving the Sensitivity of Retinoic Acids by Capillary Electrophoresis and Micro-column Solid Phase Extraction

作　　者：张普敦[1] 许国旺[1] 熊建辉[1] 郑育芳[1] 石先哲[1] 杨青[1] 魏复盛[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心,辽宁大连116011

出　　处：《分析科学学报》2004年第3期225-228,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.29975029)

摘　　要：本文发展了一种适于复杂样品预处理的微柱固相萃取装置。该装置与毛细管电泳结合用于13 顺 维甲酸和全反式 维甲酸异构体的分析,灵敏度可提高100倍以上。维甲酸异构体的微柱固相萃取过程包括柱老化、上样、洗涤、洗脱以及柱再生等步骤。将该法用于加标血浆样品中的维甲酸分析,得到满意的结果。In this paper, a micro-column solid phase extraction (MicroSPE) setup was developed. The setup consisted of a manual pump, several insulin syringes and a teflon extraction catridge filled with solid phase. 13-cis-retinoic acid and all-trans-retinoic acid isomers were analyzed by capillary electrophoresis and the MicroSPE method. The sensitivity was improved more than 100-fold. The extraction procedure included conditioning, loading, washing, eluting and regenerating. The method was applied to the analysis of retinoic acid isomers in spiked plasma and the satisfactory result was obtained.

关 键 词：微柱固相萃取 毛细管电泳 维甲酸 灵敏度 

分 类 号：O657.8[理学—分析化学] O658.2[理学—化学]