应用组织芯片技术研究硒蛋白P在肝细胞癌中的表达及意义  

Study on expression and clinical significance of selenoprotein-P in hepatocellular carcinoma by tissue microarray technique

作　　者：宁力[1] 马庆久[1] 朗兵[2] 晏伟[3] 鲁建国[1] 赖大年[1] 杨建国[4] 林晨[4] 

机构地区：[1]第四军医大学唐都医院普通外科,陕西西安710038 [2]第四军医大学基础部神经生物学教研室,陕西西安710033 [3]第四军医大学西京医院病理科,陕西西安710033 [4]中国医学科学院中国协和医科大学肿瘤研究所(医院)分子肿瘤学国家重点实验室,北京100021

出　　处：《第四军医大学学报》2004年第7期590-592,共3页Journal of the Fourth Military Medical University

摘　　要：目的 :探讨硒蛋白P(selenoprotein P ,Se P)在肝细胞癌 (hepatocellularcarcinoma ,HCC)中的表达及其临床病理意义 .方法 :分别选取HCC组织标本 84例、正常肝组织标本 6 0例制备组织芯片 .应用免疫组织化学ABC法 ,用Se P兔抗人多克隆抗体检测 84例HCC组织和 6 0例正常肝组织中Se P的表达 ,同时结合临床病理进行分析 .结果 :Se P在HCC组织和正常肝组织中阳性表达率分别为 77.4 % (6 5 / 84 )和 95 .0 %(5 7/ 6 0 ) ,二者有显著性差异 (P <0 .0 5 ) .HCC中Se P阳性率与患者肝功能Child分级有关 ,与肿瘤TNM分期、Edmondson病理分级无关 .结论 :Se P可能与HCC的发生有关 .HCC组织中SeAIM: To study the expression of selenoprotein P (Se P) in hepatocellular carcinoma (HCC) and its significance in clinical pathology. METHODS: Se P expression was detected in 84 cases of HCC tissues and 60 cases of normal liver tissues with polyclone antibody by tissue microarrys and immunohistochemistry. The results were compared with clinicopathologic grades. RESULTS: The positive expression rates of Se P in HCC and normal liver tissues were 77.4% (65/84) and 95.0% (57/60), respectively, showing significant statistical difference ( P <0.05). The Se P expression was correlated to the Child Classification. But, no correlation was found between Se P expression level, the TNM classification and the Edmondson grade. CONCLUSION: Se P may play an important role in the carcinogenesis of HCC. The expression level of Se P in HCC tissues may serve as a clinicopathologic index for the hepatic function and prognosis of HCC.

关 键 词：硒蛋白P 肝肿瘤 组织芯片 免疫组织化学 

分 类 号：R793.86[医药卫生—临床医学]