4′-羰基乙氧苯基4-胍基苯甲酸酯盐酸盐对人精子顶体蛋白酶活性的影响  

The inhibitory effect of 4′-carboethoxyphenyl 4-guanidinobenzoate hydrochloride on acrosin activity of human sperm

作　　者：袁德祥[1] 刁筱辉[1] 

机构地区：[1]天津市计划生育研究所,邮政编码300191

出　　处：《生殖医学杂志》1992年第1期38-42,共5页Journal of Reproductive Medicine

摘　　要：应用明胶底物膜方法测定了4′-羰基乙氧苯基4-胍基苯甲酸酯盐酸盐(CEGB)对生育男子精子的明胶水解(顶体蛋白酶)活性的抑制作用。实验结果表明当底物膜中 CEGB 的浓度逐渐增加时,精子的晕圈形成率(SHF)逐渐减少,平均晕圈直径(MHD)和平均溶解面积(MLA)都逐渐缩小;当CEGB 的浓度增加到6μmol/L 时则可完全抑制精子的晕圈形成。结果表明 CEGB 能够抑制人精子的明胶水解活性。In vitro study was performed to evaluate the effect of 4′-carboethoxyphenyl 4-guanidino benzoate hy- drochloride(CEGB) on acrosin activity of human sperm.A modified gelatin substrate film assay was used to test the acrosin activity,marked by a halo for- mation around the sperm head.A positive correlation exists between the size of the halo,the lysis area(halo formation) and the degree of acrosin activity.Infiltra- tion of the gelatin substrate film with proteinase in- hibitor CEGB in concertrations of 2,4,6,8,10/μmol/L before adding sperm suspension showed reduction in number of halo formations and in diameter of halo in different degrees.Halo formation was completely sup- pressed when CEGB was present at s concentration of 6μmol/L.The results indicated that a negative correla- tion exists between the concentration of CEGB and the acrosin activity(r=-0.995).

关 键 词：蛋白酶抑制剂 精子 顶体蛋白酶 CEGB 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]