β-内啡肽对体外灌流大鼠垂体前叶细胞LH分泌的抑制作用  

An inhibitory effect of β-endorphin on luteinizing hormome secretion of perif(?)sed rat anterior pituitary cells in vitro

作　　者：喻晶华 郭建[1] 郭景 屈智超 

机构地区：[1]四川省计划生育科学研究所生理室

出　　处：《生殖医学杂志》1992年第1期31-33,共3页Journal of Reproductive Medicine

基　　金：四川省计划生育委员会资助项目;WHO MRP资助项目

摘　　要：本实验应用分散大鼠垂体前叶细胞体外灌流培养方法,观察了β-内啡肽及其拮抗剂——纳洛酮对垂体细胞 LH 分泌的影响。结果表明:(1)β-内啡肽抑制了 LHRH(GnRH)-诱发的 LH 分泌,使LH 分泌峰值降低,净释放量/脉冲减少。(2)纳洛酮不仅能阻断β-内啡肽的抑制作用,还可直接增加GnRH-诱发的 LH 分泌,使 LH 峰值升高,净释放量/脉冲增加。(3)β-内啡肽及纳洛酮对 LH 的基础分泌无显著影响。本实验通过从预培养到灌流期间均用含β-内啡肽的培养液以延长作用时间,与灌流时才用β-内啡肽处理的细胞相对比,作用无明显的时间依赖关系。本研究结果为β-内啡肽在垂体水平的直接作用提供了更多的证据。Using perifusion culture of dispersed rat anterior pituitary cells,the effect of/3-endorphin,and naloxone on LH secretion was investigated.The results showed that β-endorphin reduced GnRH-induced peak LH and total LH release/pulse.Naloxone,counteracted the in- hibitory effect of β-endorphin,and a direct stimulatory effect of naloxone on GnRH-induced LH secretion was also observed.β-endorphin and naloxone did not signif- icantly affect basal LH secretion.There was no signifi- cant difference in LH secretion between pituitary cells, preincubeted with β-endorphin for 48 hours and then followed by perifusion with medium containing β-en- dorphin,and those without preincubation.The result suggested that there is no apparent time-dependent in- hibitory effect of β-endorphin.Our data demonstreted that β-endorphin can exert a direct inhibitory effect on GnRH-induced LH secretion on pituitary cells in vitro.

关 键 词：垂体前叶 内啡肽 灌注法 LH 

分 类 号：R335.1[医药卫生—人体生理学]