免疫抑制小鼠肾包膜下胃癌移植瘤模型的建立及微血管观察  

Establishment of immunosuppressed mice subrenal capsular model and examination of microvessel density

作　　者：王瑜[1] 田晶[2] 李科[3] 李艳[1] 姚敏捷[1] 

机构地区：[1]第四军医大学吉林军医学院临床检验教研室 [2]第四军医大学吉林军医学院生理学教研室,吉林吉林132013 [3]解放军306医院,北京100000

出　　处：《第四军医大学学报》2004年第6期571-573,共3页Journal of the Fourth Military Medical University

摘　　要：目的 :建立一种免疫抑制小鼠肾包膜下胃癌移植瘤模型 ,探讨其在观察肿瘤新生血管方面的意义 .方法 :环磷酰胺 (CTX)处理小鼠 ,建立免疫抑制小鼠模型 .以正常小鼠作为对照 ,用同一新鲜胃癌标本进行肾包膜下移植 ,比较两组第 7日后移植物体积和微血管密度 (Ⅷ因子抗体免疫组化染色 ) .结果 :免疫抑制小鼠移植物体积和微血管密度均高于正常免疫力小鼠 (P <0 .0 5 ) .结论 :该免疫抑制肾包膜下动物模型中移植瘤 7d内持续生长 ,微血管形成良好 ,为研究肿瘤新生血管提供了良好的模型 .AIM: To establish a mouse subrenal capsular model and to determine its roles in tumor angiogenesis. METHODS: BALB/c mice were pretreated with CTX to establish the immunosuppressed mice model and SRCA (subrenal capsular assay) was carried out simultaneously with fresh explants of human gastric cancer in two groups: the CTX immunosuppressed mice and normal mice. Microvessel density was examined by immunohistochemical staining with antihuman factor Ⅷ antibody. RESULTS: The xenografts grew constantly within 7 days after SRCA in CTX mice and the xenograft size in the CTX mice was bigger than that in normal mice ( P <0.05) and the microvessel density in the CTX mice was significantly higher than that in the normal mice( P <0.05). CONCLUSION: Mouse subrenal capsular transplantation is an acceptable short term in vivo model in the study of tumor angiogenisis. Xenografts under renal capsule of CTX immunosuppressed mice grow constantly within 7 days and angiogenisis is observed.

关 键 词：肾包膜下移植 微血管密度 免疫抑制小鼠 

分 类 号：Q781[生物学—分子生物学]