脐带血干细胞渗透特性实验研究(英文)  

Experimental Study on Osmotic Properties of Human Cord Blood Stem Cell

作　　者：张绍志[1] 王葳[1] 周新丽[1] 陈光明[1] 胡军祥[2] 王金福[2] 项盈[2] 殷华波[2] 

机构地区：[1]浙江大学制冷低温研究所,浙江杭州310027 [2]浙江大学生命科学学院,浙江杭州310027

出　　处：《生物数学学报》2004年第1期26-30,共5页Journal of Biomathematics

基　　金：Supported by Chinese National Natural Science Fund Project "Study on the mechanism of cryopreservation of biological materials by ultrasound-aided vitrification",(50076039).

摘　　要：冷冻过程中水的输运直接影响生物材料低温保存的效果。对细胞渗透特性的测量将有助于低温保存方案的设计。本文采用渗透腔法,对脐带血干细胞的渗透性能或特性进行了实验研究。实验测得脐带血干细胞的不变体积比例为0.52±0.07,平均直径为9.9±1.0μm,25℃下对水的渗透系数为4.394×10^(-13)m^3/N·s,10℃下对水的渗透系数为1.256×10^(-13)m^3/N·s。根据Arrhemius关系式,得到渗透系数的活化能为58.6κJ/mol。文中将脐带血干细胞的这些性能数据与从其它文章得到的干细胞的类似性能数据进行了对比。The transport of water during freezing directly influences the result of cryopreservation of biological materials. Measurement of the osmotic properties of cells will help the design of cryopreservation protocols. In this paper, the osmotic properties of human cord blood stem cells (HCBSCs) are experimentally studied with a diffusion-chamber method. The inactive volume ratio of HCBSCs is determined to be 0.52±0.07. The average diameter of HCBSCs is 9.9±1.0μm. The water permeability coefficients are 4.394×10-13m3/N. s at 25℃ and 1.256×10-13m3/N.s at 10℃.With an Arrhemius relationship, the activation energy is calculated to be 58.6kJ/mol. The data obtained are compared to those of stem cell from other sources.

关 键 词：干细胞 渗透特性 脐带血 低温保存 拟合 冷冻 水 

分 类 号：Q272[生物学—细胞生物学] TB112[理学—数学]