紫外线致弱童虫免疫家兔血清筛选日本血吸虫成虫cDNA文库  

Screening of cDNA Library of Schistosoma japonicum with Sera from Rabbits Vaccinated with Ultraviolet-attenuated Schistosomula

作　　者：李小红[1] 刘述先[1] 宋光承[1] 徐裕信[1] 曹建平[1] 陈家旭[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾,血吸虫病和丝虫病合作中心,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》2004年第2期80-82,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：本研究获国家 863高技术计划 (No .1 0 2 0 70 4 0 1 97)资助~~

摘　　要：目的　用致弱疫苗免疫血清、感染血清筛选文库获取新的日本血吸虫特异性未知抗原基因。　方法　用紫外线致弱童虫免疫兔血清、感染血清免疫筛选日本血吸虫成虫cDNA文库 ,对阳性重组子进行克隆、测序 ,利用软件对核酸序列进行分析 ,确定目的基因。　结果　筛选出 6种蛋白分子基因 :3 磷酸甘油醛脱氢酶 (GAPDH) ,丝氨酸蛋白酶抑制剂 (serpin) ,线粒体编码蛋白 ,肌球蛋白 (myosin)部分重链基因以及两个未知新基因。Objective To search new potential schistosomiasis vaccine by screening cDNA library with sera of rabbits vaccinated with attenuated larvae. Methods Schistosoma japonicum (Sj) adult worm cDNA library was screened with sera of rabbits vaccinated with ultraviolet-attenuated schitosomula and by sera of infected rabbits, and the inserts of positive clones were amplified and sequenced. Results Six kinds of Sj genes were obtained after three rounds of screening. Among the genes were glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors (serpin), mitochondrion coding region, part of myosin heavy chain gene and two new genes, respectively. Conclusion Screening cDNA library with sera of animals vaccinated with attenuated larvae is an effective way to search new vaccine candidates for schistosomiasis.

关 键 词：紫外线 日本血吸虫 CDNA文库 致弱疫苗 核酸序列 

分 类 号：R383.24[医药卫生—医学寄生虫学]