抗环子孢子蛋白保守II^+区单克隆抗体的制备及鉴定  

Preparation and Identification of Monoclonal Antibodies against the Region II^+ Motif in Circumsporozoite Protein of Plasmodium falciparum

作　　者：张瑞娟[1] 朱淮民[1] 李翔宇[1] 

机构地区：[1]第二军医大学病原生物学教研室,上海200433

出　　处：《中国寄生虫学与寄生虫病杂志》2004年第2期83-85,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金："十五"国家科技攻关资助项目 (2 0 0 1BA70 5B0 9)~~

摘　　要：目的　获得针对恶性疟原虫环子孢子蛋白 (CSP)保守II+ 区 (RegionII+ )的单克隆抗体。　方法　采用恶性疟原虫保守II+ 区十二肽 (EWSPCSVTCGNG)免疫BALB/c小鼠 ,经融合 ,ELISA 3次筛选 ,获得 3株分泌针对保守II+ 区单克隆抗体的杂交瘤细胞株。　结果　ELISA检测结果显示单抗能与重组表达的恶性疟原虫CSP片段及天然CSP特异性反应。间接荧光抗体检测显示 ,单抗不仅能识别恶性疟原虫子孢子 ,也能识别约氏疟原虫子孢子。　结论　成功地获得了针对恶性疟原虫CSP保守II+Objective To develop and identify the monoclonal antibodies (McAbs) against Region II^+ motif in circumsporozoite protein of Plasmodium falciparum. Methods BALB/c mice were immunized with 12 peptides within Region Ⅱ^+ in circumsporozoite protein of P. falciparum. Spleen cells isolated from the immunized mice were fused with myeloma cell. After three times screening with ELISA, 3 positive hybridoma cell lines were obtained. Results ELISA test indicated that the McAbs reacted with recombinant circumsporozoite protein fragment containing tandemly repeat region and conserved Region II^+. IFA test showed that the McAbs recognized not only the sporozoites of P. falciparum, but also the sporozoifes of P. yoelii. Conclusion McAbs obtained can probe the Region II^+ motif in circumsporozoite protein of P. falciparum, which might also recognize that of other Plasmodium species.

关 键 词：疟原虫属 单克隆抗体 环子孢子蛋白 ELISA 疟疾 

分 类 号：R382.31[医药卫生—医学寄生虫学]