超抗原SEA对Tc1和Tc2细胞分化和增殖的影响  

The different influence of SEA on the differentiation and proliferation of Tc1 and Tc2 subsets

作　　者：韩卫宁[1] 张赟[1] 曹云新[1] 李琦[1] 庄然[1] 刘雪松[1] 金伯泉[1] 

机构地区：[1]第四军医大学免疫学教研室,西安710032

出　　处：《中华微生物学和免疫学杂志》2004年第4期300-303,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目 (3 0 0 0 0 15 0 )

摘　　要：目的　探讨超抗原SEA对Tc1和Tc2亚群细胞增殖的不同影响。方法　以SEA刺激PBMC ,以免疫荧光染色结合流式细胞术比较刺激与未刺激的PBMC中T细胞各亚群比例的变化 ;从PBMC中分离CD8+T细胞 ,定向诱导为Tc1和Tc2细胞系 ,3 H TdR掺入法检测Tc1和Tc2细胞对SEA刺激的反应 ,用免疫荧光染色和流式细胞术检测CD2 5的表达。结果　经SEA刺激后 ,PBMC中的Tc0 /TH0增殖并向Ⅰ型或Ⅱ型分化 ,但SEA所诱导PBMC中的Tc2 /TH2的比例远高于Tc1/TH1;SEA在体外能更有效地促进Tc2亚群细胞的增殖 ;经SEA刺激的Tc2细胞系CD2 5的表达也高于Tc1。结论　SEA优先诱导PBMC中的Tc2 /TH2的分化 。Objective To examine the influence of the superantigen Staphylococcus enterotoxin A (SEA) on the differentiation and proliferation of Tc1 and Tc2 cell line. Methods The PBMCs were stimulated by SEA, and the ratio of Tc1/Tc2 in PBMC stimulated with or without SEA or not were compared by immunofluorescence staining and flow cytometry analysis. CD8 +T cells were purified and inducd to Tc1 or Tc2 subsets and the discrepancy between the extent of Tc1 or Tc2 proliferation were tested by 3H-TdR incorporation. The expression of CD25 on Tc1 or Tc2 cell line were determined by immunofluorescence staining and flow cytometry analysis. Results Stimulated by SEA, Tc0 or T H0 in PBMC proliferated and polarized to typeⅠor Ⅱ T cell, and the percentage of Tc2/T H2 was higher than that of Tc1/T H1. SEA could efficiently promote Tc2 cell proliferation in vitro and the expression level of CD25 on Tc2 was higher than that on Tc1 cells. Conclusion SEA preferentially induced the differentiation of Tc2/T H2 and could efficiently promote Tc2 cell proliferation in vitro .

关 键 词：金黄色葡萄球菌外毒素A 细胞毒性T细胞 超抗原 细胞分化 流式细胞术 免疫荧光染色法 

分 类 号：R392[医药卫生—免疫学]