11,12-EET长时间保存未成熟离体兔心的初步研究  

Experimental study of prolonged preservation of immature isolated rabbit hearts with 11,12-epoxyeicosatrienoic acid

作　　者：周明阳[1] 吴清玉[1] 钟星[2] 龙村[3] 温复兴[3] 覃筱燕[4] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院心外科,北京100037 [2]中国医学科学院肿瘤研究所 [3]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院麻醉与体外循环研究室,北京100037 [4]中央民族大学生物化学系

出　　处：《中华胸心血管外科杂志》2004年第1期10-12,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

摘　　要：目的　探讨 11,12 EET(11,12 epoxyeicosatrienoicacid)对未成熟兔离体心脏缺血再灌注损伤的保护作用及机制。方法　利用非循环式Langendorff灌注装置将 16只未成熟离体兔心制成缺血再灌注模型 ,随机分成对照组 (St.ThomasNo 2液为停搏液及保存液 )和实验组 (St.ThomasNo 2液加 11,12 EET为停搏液及保存液 )。测定心脏保存 (4°C) 8h、再灌注 (37°C) 30min的心功能指标 :左心室变化压、舒张末压、最大压力变化速率、冠状动脉流量以及心肌含水量、心律失常评分和心肌酶CK、LDH、心肌超微结构改变等。结果　 (1)实验组的心功能恢复率、心肌水肿程度、心肌超微结构和心肌酶及心律失常评分改变均优于对照组。 (2 )与保存前相比 ,实验组的心功能无显著性改变 ,心功能恢复基本接近保存前水平。结论　St.ThomasNo 2液中加入 11,12 EET可增强对未成熟兔离体心脏的保存效果。Objective: To investigate the protective effect of 11,12-EET(11,12-epoxyeicosatrienoic acid)on myocardium of immature rabbit hearts from ischemic reperfusion injury. Methods: 16 isolated immature rabbit hearts were performed to ischemic reperfusion model in a Langendorff perfusion apparatus and randomlyassigned to on two groups. Control group, the hearts were arrested with St.Thomas No.2 solution and stored in the same solution (n=8). EET group, the hearts were arrested with St.Thomas No.2 plus 11,12-EET solution and stored in the same solution (n=8). These isolated rabbit hearts were stored for 8 hours at 4℃ hypothermia , and underwent 30 minutes of reperfusion (37℃). We measured the preischemia and postreperfusion indexes of left ventricle developed pressure (LVDP), left ventricle end-diastolic pressure (LVEDP), ±dp/pt_ max , myocardial water content (MWC), coronary blood effluent (CBE) and arrhythmia score (AS). The myocardial ultrastructure and value of creatine kinase (CK) and lactic dehydrogenase (LDH) were also observed. Results: (1) After 30 minutes reperfusion, the indexes of CK, LDH, CBE, AS,and the recovery rate of heart function were significantly better in EET group compared with controls. At the same time, no ultrastructural changes were found in the EET group while the capillary endothelial base membrane edema and mitochondrion edema was observed in the control group. (2) In EET group, compared with preischemia, there were no significantly changes of myocardial function at the end of 30-minutes reperfusion. Conclusion: These data suggest that 11,12-EET add to the St.Thomas No.2 solution could offer more little myocardial injury and little arrhythmia and provide better preservation of the isolated immature hearts.

关 键 词：11 12-EET 长时间保存 未成熟离体兔心 心麻痹液 再灌注损伤 心脏保护 心脏缺血 心脏移植术 

分 类 号：R654.2[医药卫生—外科学]