热休克蛋白90在门脉高压大鼠内脏高动力循环中的作用  

Role of heat shock protein 90 in hyperdynamic circulation of portal hypertensive rats

作　　者：艾建华[1] 杨镇[1] 裘法祖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科

出　　处：《中华医学杂志》2004年第1期9-13,共5页National Medical Journal of China

基　　金：国家自然科学基金资助项目 ( 3 0 170 92 0 )

摘　　要：目的　研究热休克蛋白 90 (HSP90 )在门脉高压大鼠内脏高动力循环中的作用。方法2 0只SD大鼠制成门脉高压动物模型 ,1 0只大鼠行假手术作为对照。 4周后处死动物获取肠系膜动脉。测定其对甲氧胺 (MTX)和乙酰胆碱的量效曲线。然后再灌注格尔德霉素 (GA) ,再次测量其量效曲线。去内膜后 ,再测量肠系膜动脉对硝普钠 (SNP)的量效曲线。结果　肠系膜动脉对MTX的反应性降低。而GA能增强门脉高压大鼠肠系膜血管对MTX的反应性。GA还能减少肠系膜血管乙酰胆碱依赖的血管扩张 ,但不能减少SNP依赖的血管扩张。Objective To study the role of heat shock protein 90 (HSP 90) in the formation of hyperdynamic circulation of portal hypertensive rats. Methods Animal model of portal hypertension was established by partial ligation of the portal vein among 20 SD rats (group P). Ten rats underwent sham operation (group S) Four weeks after the operation, the rats were killed and their mesenteric arteries and portal veins were obtained. The expression of HSP 90 in the portal veins and part of the superior mesenteric arteries was detected by Western blotting. Another mesenteric arteries underwent continuous perfusion with methoxamine (MTX, 30 and 100 μmol/L) or acetylcholine (Ach). The perfusion pressure was monitored by a sensor. The concentration response curves were examined in response to MTX and Ach infusion respectively. Then geldanamycin (GA) was infused; responses to MTX and ACh were repeated as described above. The endothelia of these arteries was denuded. After endothelial denudation was achieved, response to sodium nitroprusside (SNP) was examined. Results The expression of HSP 90 was much stronger in the group P than in the group S. The perfusion pressure of the mesenteric artery of the group P was 13 32 mm Hg±0 55 mm Hg, significantly lower than that of the group S (17 33 mm Hg±0 57 mm Hg, n =10, P <0 001) After the addition of MTX, the perfusion pressure of the mesenteric artery of the group P was 141 9 mm Hg±7 6 mm Hg, significantly lower than that of the group S (181 1 mm Hg±16 2 mm Hg, n =8, P <0 001) GA and 30 μmol/L or 100 μmol/L MTX were added successively into the perfusion fluid for the mesenteric superior artery of the group P, then the perfusion pressure became 106 7 mm Hg±7 5 mm Hg or 124 9 mm Hg±5 5 mm Hg, significantly higher than those of the mesenteric superior artery of the group P without addition of GA (60 9 mm Hg±4 3 mm Hg, n =5, P <0 05 and 98 7 mm Hg±4 7 mm Hg, n =5, P <0 05) GA and 100 μmol/L MTX were added successively into the

关 键 词：热休克蛋白90 门脉高压症 大鼠 内脏高动力循环 动物模型 甲氧胺 乙酰胆碱 

分 类 号：R657.3[医药卫生—外科学]