核小体诱导BALB/c小鼠产生狼疮肾的研究  

Nucleosome Induced Systemic Lupus Erythematosus-like Disease in BALB/c Mice

作　　者：郝进[1] 郝飞[1] 钟白玉[1] 唐书谦[1] 潘峰[2] 苟俊[2] 徐云升[1] 

机构地区：[1]第三军医大学西南医院皮肤性病科,重庆400038 [2]第三军医大学基础部中心实验室

出　　处：《中华皮肤科杂志》2004年第1期12-14,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助(30200258)

摘　　要：目的了解核小体在系统性红斑狼疮(SLE)发病中的作用。方法用蔗糖连续密度梯度离心法从新鲜鸡血红细胞中提取核小体,皮下免疫BALB/c小鼠,采集小鼠血清和肾脏标本。ELISA方法检测其IgG型dsDNA抗体和ANA抗体水平;直接免疫荧光检测小鼠肾脏IgG型免疫复合物的沉积情况;组织病理观察小鼠肾脏损伤情况。结果核小体免疫后14d,BALB/c小鼠血清中开始出现IgG型dsDNA抗体和ANA抗体,其滴度随时间延长逐渐升高;核小体免疫后35d,小鼠肾脏出现大量IgG型免疫复合物沉积,光镜观察显示有明显肾脏损伤。对照组小鼠无明显自身抗体产生和肾脏病变。结论核小体可以诱导正常小鼠产生SLE样征候群,提示核小体参与SLE的发病。Objective To investigate the role of nucleosome in the pathogenesis of systemic lupus erythematosus (SLE). Methods BALB/c mice were immunized with nucleosome, and then serum dsDNA and ANA autoantibodies were detected by ELISA. Kidney specimens were observed by immunofluorescence and histological examination. Results High titers of IgG dsDNA and ANA autoantibodies in sera of BALB/c mice were observed at the 14th day after immunization with nucleosome. Nephritis and immune complex deposition in renal glomeruli were observed at the 35th day. Conclusion Nucleosome could induce SLE-like disease in BALB/c mice, and may play a critical role in the pathogenesis of SLE.

关 键 词：核小体 BALB/C小鼠 系统性红斑狼疮 肾脏损伤 并发症 检测 

分 类 号：R593.242[医药卫生—内科学]