系统性红斑狼疮患者抗磷脂抗体的检测  

Detecting Six Kinds of Antiphospholipid Antibodies in Serum from 32 Patients with Systematic Lupus Erythematosus

作　　者：郭哲[1] 赵玉铭[1] 王雅坤[1] 赖鑫[1] 陈洪铎[1] 

机构地区：[1]中国医科大学附属第一医院皮肤科

出　　处：《中华皮肤科杂志》2004年第1期27-28,共2页Chinese Journal of Dermatology

摘　　要：目的探讨抗磷脂抗体(APA)在正常人的分布及其与系统性红斑狼疮(SLE)的关系。方法应用酶联免疫吸附实验(ELISA)检测82例正常人和32例SLE患者血中6种APA。结果正常人IgG型抗心磷脂抗体(aCL)、抗磷脂酰肌醇抗体(aPI)、抗磷脂酸抗体(aPA)、抗磷脂酰乙醇胺抗体(aPE)、抗磷脂酰丝氨酸抗体(aPS)和抗磷脂酰胆碱抗体(aPC)的阳性率分别为6.10%、6.10%、7.32%、7.32%、3.66%和3.66%,IgM型aCL、aPA、aPC、aPS、aPE和aPI的阳性率分别为2.44%、3.66%、3.66%、6.10%、6.10%和4.88%。SLE患者IgG型aCL、aPA、aPS、aPC、aPI的A值和阳性率显著高于正常人,IgM型aCL、aPA、aPS、aPE、aPC的A值和阳性率显著高于正常人;SLE时IgG和IgM型APA的总阳性率分别为68.75%和71.88%。结论在正常人中存在滴度较低的APA;这些APA互相间有一定的关系,多同时出现。SLE时进行APA检查,对抗磷脂综合征的诊断可能有一定的意义。Objective To investigate the distribution of antiphospholipid antibodies(APA) in patients with systematic lupus erythematosus (SLE) and normal controls and its significance. Methods Six kinds of APA were detected by enzyme linked immunosorbent assay(ELISA). The examined sera were collected from 82 cases of normal controls and 32 cases of SLE. Results ①The positivity rate of IgG type aCL, aPI, aPA, aPE, aPS and aPC in the serum from normal controls was 6.10%, 6.10%, 7.32%, 7.32%, 3.66% or 3.66%, respectively. ②The positivity rate of IgM type aCL, aPA, aPC, aPS, aPE aPI in serum from normal controls was 2.44%, 3.66%, 3.66%, 6.10%, 6.10% and 4.88%, respectively. ③Compared with normal controls, the A values and positivity rate of IgG type of aCL, aPA, aPS, aPC and aPI in SLE patients were significantly higher. ④Compared with normal controls, the A values and positivity rate of IgM type of aCL, aPA, aPS, aPE and aPC in SLE patients were significantly higher. ⑤The total positivity rate of IgG type and IgM type APA was 68.75% or 71.88%, respectively. Conclusion ①There is lower titer of APA in normal controls, with some relationships between different types of APA and they appear at the same time.②the measurment of APA in sera of SLE might be benefit to the diagnosis of antiphospholipid syndrome. [

关 键 词：系统性红斑狼疮 抗磷脂抗体 检测 酶联免疫吸附实验 抗磷脂酰乙醇胺抗体 抗磷脂酰肌醇抗体 

分 类 号：R593.241[医药卫生—内科学] R446.62[医药卫生—临床医学]