梭形双通道桥接管的研制及其桥接大鼠坐骨神经缺损的研究  

Development of fusiform double channel nerve conduit and its role in bridging the sciatic nerve gap of rats

作　　者：李强[1] 伍亚民[1] 李民[1] 刘媛[1] 杨恒文[1] 李应玉[1] 曾琳[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所第三研究室

出　　处：《中华创伤杂志》2004年第4期230-233,共4页Chinese Journal of Trauma

基　　金：国家重点基础研究发展规划资助项目(G19990 5 42 0 6)

摘　　要：目的　通过研制新型的梭形双通道桥接管建立坐骨神经缺损修复的理想实验研究模型 ,评估该模型的科学性及可行性。　方法　利用医用乳胶管制成梭形双通道桥接管 ,桥接管以中轴左右对称 ,垂直长度为 10mm ,管外径 2mm ,内径 1.8mm。用其桥接 6 0只SD大鼠坐骨神经缺损 ,将大鼠随机分为三组 ,每组 2 0只 ,A组 :空白对照 ,B组 :医用几丁糖凝胶 10 0 μl;C组 :医用几丁糖凝胶 10 0 μl+2 0ng/ μl神经生长因子 (NGF) 5 μl和医用几丁糖凝胶 10 0 μl+6 0ng/ μl睫状神经营养因子 (CNTF) 5 μl。通过大体形态和组织病理学观察坐骨神经的再生情况。　 结果　桥接管无移位、变形、塌陷及裂开 ,且桥接管周围组织炎性反应轻 ,病理学检查为网状纤维和新生血管 ;术后 4 ,8,12周时 ,A组和B组两支管内再生神经大体形态和组织病理学无显著性差异 ,而C组CNTF侧再生纤维明显优于NGF侧。　结论用新型的梭形双通道桥接管桥接大鼠坐骨神经缺损具有科学性和可行性 。Objective To design and manufacture fusiform double channel nerve conduit (FDCNC) aiming at creating an ideal experimental model to bridge the sciatic nerve gap of rats and evaluate the feasibility and application of FDCNC. Methods The new symmetry FDCNC was made of the medical latex tubing. The nerve conduit was 10 mm long in vertical with outside diameter of 2 mm and inside diameter of 1.8 mm. Sixty rats with sciatic nerve injury bridged with FDCNC were randomly and equally divided into three groups, ie, Group A (blank control group), Group B (100 μl medical chitosan jel was injected into each branch of the conduit) and Group C, infused with 100 μl medical chitosan jel plus 5 μl nerve growth factor (NGF) at 20 ng/μl, and 100 μl medical chitosan jel plus 5 μl ciliary neurotrophic factor (CNTF) at 60 ng/μl. The regenerative sciatic nerves were observed histopathologically and morphologically 4, 8 and 12 weeks respectively after injury. Results FDCNC showed no any malposition, deformation or breakage. The inflammatory reaction of tissues around FDCNC was slight. The histological examination showed mainly reticular fiber vessels or new vessels. The regenerative nerves showed no significant differences in histopathology and morphology in the Groups A and B. But in the Group C, the regenerative nerve in branch contained CNTF was better than that in branch contained NGF. Conclusion The new FDCNC is feasible and reasonable to bridge the sciatic nerve gap as well as a satisfactory model for study of the peripheral nerve injury.

关 键 词：梭形双通道桥接管 大鼠 坐骨神经缺损 神经生长因子 医用几丁糖凝胶 组织病理学 

分 类 号：R651.1[医药卫生—外科学]