复合益生菌活菌制剂对肝硬化患者肠道菌群的影响  

The effects of compound probiotics on intestinal microflora in patient with liver cirrhosis

作　　者：邹志强 宋波[2] 

机构地区：[1]烟台市传染病医院,山东烟台264001 [2]烟台毓璜顶医院消化内科

出　　处：《中国微生态学杂志》2004年第2期97-97,99,共2页Chinese Journal of Microecology

摘　　要：目的 :观察肝硬化患者口服三联活菌后肠道菌群、血氧及血浆内毒素的变化。方法 :选择肠道菌群中具有代表性的细菌进行培养和计数。 4 2例肝硬化患者给予三联活菌治疗 2 1d。测定治疗前后肠道菌群菌落计数、血氨 (干片法 )、血浆内毒素 (改良鲎试验法 )。结果 :与正常对照组相比 ,肝硬化组患者存在不同程度的肠道菌群失调 ,主要表现为双歧杆菌减少 (10 12± 0 71比 9 2 7± 1 2 5 ,P <0 0 5 )。治疗后 ,双歧杆菌由 9 2 7± 1 2 5增至 10 4 3± 1 2 5 ,差异有显著性 (P <0 0 1)。且血氨水平降低 ,并可降低肝硬化患者血浆内毒素水平 [(10 9 2 1± 12 2 3) pg/ml比 (71 4 6± 9 12 ) pg/ml,P <0 0 5 ]。 结论 :肝硬化患者存在肠道菌群失调。益生菌制剂可有效改善肝硬化患者肠道菌群失调 ,并降低血氨及血浆内毒素。Objective:To investigate the changes in intestinal flora,blood ammonia and serum endotoxin after the administration of probiotics.Methods:Six bacteria were cultured and the colony forming uints were counted in the stool.Forty-two patients were randomized to receive probiotics capsules contains Bifidobacterrium,Lactobacillus acidophilus and Enterococcus for 21 days.Fecal flora,blood ammonia (detected by test paper) and serum endotoxin(detected by limulus ameobocyte lysate test kits) were measured before and after the treatment.Results:Patients with liver cirrhosis had varying degrees of intestinal flora imbalance,shown by a decrease in the count of Bifidobacterium(10.12±0.71 vs.9.27±1.25,P<0.05).Probiotics could increase Bifidobacterium count (9.27±1.25 vs 10.43±1.25,P<0.01) and reduce the level of blood ammonia and serum endotoxin[(109.21±12.23)pg/ml vs(71.46±9.12) pg/ml,P<0.05].Conclusion:Patients with liver cirrhosis have imbalance of intestinal flora.probiotics effectively invrease the Bifidobacterium count and reduce the level of blood ammonia and serum endotoxin in patients with liver cirrhosis.

关 键 词：益生菌 活菌制剂 肝硬化 肠道菌群 菌群失调 

分 类 号：R378[医药卫生—病原生物学] R575[医药卫生—基础医学]