蛋白激酶C在双歧杆菌预防大肠癌生长中的作用  

The role of protein kinase C on Bifidobacterium preventing from the growth of large bowel carcinoma

作　　者：王建红 王立生 朱惠明 潘令嘉[2] 马晓东[2] 张亚历[2] 周殿元[2] 

机构地区：[1]暨南大学医学院第二附属医院深圳市人民医院,广东深圳518020 [2]第一军医大学南方医院消化科,广东广州510515

出　　处：《中国微生态学杂志》2004年第2期65-66,共2页Chinese Journal of Microecology

基　　金：广东省自然科学基金资助项目 (9940 66)

摘　　要：目的 :从信号分子蛋白激酶C(PKC)这一角度探索青春型双歧杆菌预防大肠癌生长的途径。方法 :建立大肠癌裸鼠移植瘤模型 ,用激光共聚焦显微镜测定大肠癌组织PKCα、βⅠ、βⅡ、γ、ε和 ζ的含量。结果 :荷瘤鼠经双歧杆菌预先处理后 ,其大肠癌组织PKCα和 βⅡ的平均荧光强度明显低于肿瘤对照组 (P<0 0 1) ,而PKCβⅠ、γ、ε和 ζ的平均荧光强度在两组间差异无显著性 (P >0 0 5 )。结论 :青春型双歧杆菌体内可通过降低PKCα、βⅡ的活性来预防大肠癌的生长。Objective:To explore the pathways of Bifidobacterium adolescentis preventing from the growth of large bowel carcinoma in view of the signal molecule protein kinase C(PKC).Methods:The animal model of large bowel carcinoma transplantation tumor was built.The content of PKC α?βⅠ?βⅡ?γ?ε?ζ in large bowel carcinoma tissues was detected by using laser scanning confocal microscopy.Results:After the nude mice were pretreated with Bifidobacterium,the average fluorescent strength of PKC α?βⅡ of large bowel carcinoma tissues in Bifidobacterium prevention group was markedly lower than that in tumor control group(P<0.01).The average fluorescent strength of PKC βⅠ?γ?ε and ζ was not significant difference in the two group (P>0.05).Conclusion:Bifidobacterium adolescentis could prevent from the growth of large bowel carcinoma by decreasing the activity of PKC α and PKC βⅡin vivo.

关 键 词：蛋白激酶C 双歧杆菌 预防 大肠癌 生长 荧光强度 

分 类 号：R378.992[医药卫生—病原生物学]