HPLC法测定脑立苏颗粒中原儿茶醛的含量  

Determination of protocatechualdehyde in Naolisu granules by HPLC

作　　者：都娟[1] 彭荣珍[1] 蒋媛媛[2] 

机构地区：[1]暨南大学医学院第六附属医院/江门市五邑中医院 [2]江门市中医药学校,广东江门529000

出　　处：《广东药学院学报》2004年第2期130-131,共2页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的:建立脑立苏颗粒中原儿茶醛含量测定的方法。方法:用HPLC法测定脑立苏颗粒中原儿茶醛的含量,用Shim-pack VP-ODS(4.6 mm×250 mm)柱,以ψ(甲醇:0.5％冰醋酸溶液)=15:85为流动相,检测波长280 nm,进行含量测定。结果:原儿茶醛在4.16～20.8μg/mL范围内呈良好的线性关系(r=0.9999),平均回收率为98.9％,RSD=1.44％(n=6)。结论:该方法准确、灵敏、重复性好。Objective: To establish the content determination method of protocatechualdehyde in Naolisu gradules by HPLC. Methods: HPLC was performed to determine protocatechualdehyde on ODS column at 40℃ ,the mobile phase was composed of ψ ( methanol : 0. 5% acetic acid ) =15 : 85 and detection wavelength of 280 nm. Results: The linearity of this method was good (r = 0. 9999) , with the average recovery of 98. 9% , RSD = 1. 44% ( n= 6 ) . Conclusions: This method was simple, sensitive and accurate,and applicable for quality control of Naolisu grandules.

关 键 词：脑立苏颗粒 原儿茶醛 高效液相色谱法 成分分析 

分 类 号：R286[医药卫生—中药学]