百蕊草的化学成分研究  

Study on chemical constituents of Thesium chinensis

作　　者：鲁云霞[1] 汪俊松[2] 

机构地区：[1]安徽医科大学生化教研室,安徽合肥230032 [2]中国科学院昆明植物研究所植物化学室,云南昆明650204

出　　处：《中草药》2004年第5期491-493,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的　研究百蕊草 Thesium chinense的化学成分。方法　采用反复柱色谱和制备性 HPL C百蕊草的醋酸乙酯萃取物中分离得到 5种化合物 ,通过理化和光谱分析鉴定其化学结构 ,并研究其抗氧化活性。结果　自百蕊草的醋酸乙酯萃取物中分离得到 5种黄酮苷类化合物 ,分别鉴定为 :山柰素 - 3- O-葡萄糖苷 (kaem pferol- 3- O- glucoside, ) ,柚皮素 - 4 - O-葡萄糖苷 (naringenin- 4 - O- glucoside, ) ,芹菜素 - 7- O-葡萄糖苷 (apigenin- 7- O- glucoside, ) ,木犀草素 - 7- O-葡萄糖苷 (luteolin- 7- O- glucoside, ) ,芸香苷 (rutinoside, )。抗氧化实验结果表明 ,化合物 的抗氧化作用最强 ,3个浓度中以 1× 10 - 4mol/ L 作用最明显。结论　化合物 具有较强的抗氧化作用。ObjectTo study the chemical constituents of Thesium chinensis Turcz. and their antioxidation effects. MethodsIsolation and purification were repeatedly carried out on gel column chromatography and preparative HPLC. The structures were identified and elucidated by physicochemical properties and spectral analysis. Results Five compounds were elucidated as: kaempferol-3-O-glucoside (Ⅰ), naringenin-4-O-glucoside (Ⅱ), apigenin-7-O-glucoside (Ⅲ), luteolin-7-O-glucoside (Ⅳ), rutinoside (Ⅴ). It was found that kaempferol-3-O-glucoside (Ⅰ) was the strongest one in the antioxidation experiment, its optimum concentration was 1 × 10 -4 mol/L. ConclusionCompound Ⅰ is the main antioxidation component isolated from T. chinensis.

关 键 词：百蕊草 黄酮苷 抗氧化 

分 类 号：R284.1[医药卫生—中药学]