抗EB病毒口服液对EB病毒抗原表达的抑制作用及其细胞毒作用  

Inhibition of Anti-EB Virus Liquor on EB virus antigen expression and its cytotoxicity

作　　者：刘宗潮[1] 简少文[1] 李华忠 欧景才 谢冰芬[1] 李端[1] 冯公侃[1] 

机构地区：[1]中山大学肿瘤防治中心,广东广州510060 [2]广东省177医院,广东广州510317

出　　处：《中草药》2004年第5期542-545,共4页Chinese Traditional and Herbal Drugs

基　　金：国家"十五"科技攻关计划重大项目基金资助 ( 2 0 0 1BA70 1A0 7-10 );中山大学重点学科课题基金联合资助 ( 980 96)

摘　　要：目的　观察抗 EB(Epstein- Barr)病毒口服液对 EB病毒抗原表达的影响及对鼻咽癌 CNE2 细胞的细胞毒作用。方法　用间接荧光法测定抗 EB病毒口服液对 Raji细胞早期抗原 (early antigen,EA)表达及 B95- 8细胞病毒壳抗原 (virus capsid antigen,VCA)表达的影响 ;用 MTT法测定其对鼻咽癌 CNE2 细胞的细胞毒作用。结果　抗EB病毒口服液在无毒的浓度下 ,对 Raji细胞 EB病毒 EA抗原表达有较强的抑制作用 ,其 IC50 值为 0 .6 6 7m g/m L;对 B95- 8细胞 EB病毒 VCA抗原表达有较强的抑制作用 ,其 IC50 值为 0 .89mg/m L;对正丁酸钠激发的 B95- 8细胞 EB病毒 VCA抗原表达亦有较强的抑制作用 ,其 IC50 为 1.4 mg/m L。抗 EB病毒口服液对人鼻咽癌细胞 CNE2的 IC50 为 7.5 7mg/m L。结论　抗 EB病毒口服液能抑制 EB病毒抗原的表达 ,在较高的浓度下对鼻咽癌细胞CNE2 具细胞毒作用。Object To study the inhibitory effect of Anti-EB Virus Liquor (AEVL) on EB (Epstein-Barr) virus antigen expression and its cytotoxicity. Methods The effect of AEVL on Raji cell early antigen (EA) expression and B 95-8 cell virus capsid antigen (VCA) expression was assayed by indirect fluorescent technique; the cytotoxicity on nasopharyngeal carcinoma CNE 2 cells was determined by MTT method. Results At the non-toxic concentration, AEVL had markedly inhibitory effect on Raji cell EB-virus, IC 50 was 0.667 mg/mL and showed powerfully inhibitory effect on B 95-8 cell EB-virus VCA expression, IC 50 was 0.89 mg/mL; and it had strongly inhibitory effect on B 95-8 cell EB virus VCA expression stimulated by sodium n-butyric acid, IC 50 was 1.4 mg/mL. The IC 50 of AEVL on human nasopharyngeal carcinoma CNE 2 cell was 7.57 mg/mL. Conclusion AEVL could inhibit EB virus antigen expression and have cytotoxicity on nasopharyngel carcinoma cells at high concentration.

关 键 词：抗EB病毒口服液 EB病毒 抗原表达 鼻咽癌 

分 类 号：R285.5[医药卫生—中药学]