鸡血藤高效液相色谱指纹图谱研究  

Study on HPLC fingerprint of Caulis Spatholobi

作　　者：严启新[1] 李萍[1] 

机构地区：[1]中国药科大学生药学教研室,江苏南京210038

出　　处：《中草药》2004年第5期556-559,共4页Chinese Traditional and Herbal Drugs

基　　金：国家重点科技攻关项目 ( 99-92 9-0 1-3 1)

摘　　要：目的　建立鸡血藤的高效液相色谱指纹图谱 ,以评价鸡血藤的质量。方法　用 HPL C法获得鸡血藤药材的色谱图 ,再用聚类分析方法进行考察。结果　鸡血藤共有 11个峰 ,其中非共有峰 6个 ,共有峰 5个 ,共有峰的相对保留时间分别为 :0 .0 4 6 ,0 .6 6 4 ,1.0 0 0 ,1.198,1.383;相对峰面积分别为 :0 .819,0 .2 2 1,1.0 0 0 ,0 .331,0 .4 5 8。鸡血藤及其混淆品种的色谱指纹图谱存在明显差异 ,所提取的 11个色谱峰可作为指纹特征。结论　 HPL C色谱指纹图谱分析技术可用于鸡血藤药材的鉴别。ObjectTo establish HPLC fingerprint for the identification and evaluation of Caulis Spatholobi. Methods HPLC system was used to obtain the chromatograms of Caulis Spatholobi and the clustering analysis was applied for data analysis. Results There are 11 peaks in total, among them five are common, the relative retention time is 0.046, 0.664, 1.000, 1.198, 1.383, the relative peak area is 0.819,0.221,1.000,0.331,0.458. There was obvious difference among chromatographic fingerprints of Caulis Spatholobi and its easily-confused species. The 11 measured peaks could be used as the fingerprint features. Conclusion Chromatographic fingerprint of HPLC can be used for identifying the Caulis Spatholobi and its easily-confused species.

关 键 词：鸡血藤 高效液相 指纹图谱 

分 类 号：R282.710.03[医药卫生—中药学]