创面愈合过程中脂肪细胞内转化生长因子-β各亚型的表达特点及其生物学意义  

The biologic significance of TGF-β isoforms expression in adipocyte during wound healing

作　　者：程飚[1] 付小兵[2] 刘文忠 方利君[2] 孙同柱[2] 盛志勇 

机构地区：[1]广州军区广州总医院整形外科,510010 [2]解放军第304医院全军创伤修复重点实验室

出　　处：《中华实验外科杂志》2004年第3期360-362,共3页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究发展规划资助项目(G1999054204);国家杰出青年基金资助项目(39525024);国家自然科学基金重点项目(30230370);国家自然科学基金资助项目(30170966);博士后基金(中博基[2001]No.14)

摘　　要：目的　观察深Ⅱ度烫伤大鼠上皮组织修复过程中皮下脂肪组织内脂肪细胞转化生长因子 (TGF) β1、2、3亚型的表达特点及生物学意义。 方法　将 3 6只Wistar大鼠随机分为正常对照组 (A组 ,n =6)和单纯烫伤组 (B组 ,n =3 0 )。利用大鼠 5 %深Ⅱ度烫伤模型 ,于伤后 1、3、7、14和2 1d采取全层创面皮肤标本 ,采用免疫组织化学染色法检测真皮下脂肪细胞TGF β1、2、3的表达 ,并以病理学染色观察肉芽组织的形成及创面的收缩情况。结果　正常皮肤组织内脂肪细胞中均有TGF β1、2、3的表达。单纯烫伤创面愈合的过程中 ,伤后 1d ,脂肪组织内脂肪细胞膜表面TGF β1表达较弱 ,但TGF β2、3的表达较强 ,伤后 3d ,脂肪细胞TGF β1表达有所增强 ,TGF β2、3的表达进一步增强。此时创面深层脂肪组织内有新的微小血管形成。至 14d ,创面收缩 ,脂肪组织TGF β1表达维持在较高水平 ,TGF β2、3的表达也较高。真皮浅层大量的微小血管形成 ,密度明显增强。 2 1d创面完全愈合 ,在新愈合组织内有一定量的血管形成 ,TGF β各亚型的表达有所减弱 ,但TGF β2、3的表达仍强于TGF β1。 结论　组织修复过程中 ,脂肪细胞释放的TGF β可能有助于肉芽组织充填和微小血管网的形成 ,在创面愈合中扮演重要角色。Objective To observe the changes of the expression of TGF-β isoforms in adipocytes during wound healing,and explore the biologic significance.Methods The samples were obtained from Wistar mice undergoing scald,and the samples of control from the unjuried skin.Thirty-six male Wistar rats were divided randomly into three groups as follows:(1) Normal control group (n=6);(2) Injured group:further divided into subgroups of 1,3,7,14 and 21 days after burn (n=6 each).Immunohistochemistry staining technique was employed to detect the expression of TGF-β1,2,3 monoclonal antibodies in the adipocytes of subcutaneous tissue.Results There was weak expression of TGF-β1,2,3 in the adipocytes of normal subcutaneous tissue.The expression of TGF-β isoforms was up-regulated after burn.During the wound healing,the expression of TGF-β isoforms was enhanced over time,especially the changes of TGF-β2,3 were more significant.The vascular density was enhanced.Conclusion The expression of TGF-β isoforms might have some relations to the process of revascularization.Adipocytes have garnered new attention for its excretion abilities as an endocrine organ which might play an important role in the process of wound healing.

关 键 词：创面 愈合 脂肪细胞 转化生长因子-Β 表达特点 

分 类 号：R641[医药卫生—外科学]