电针预处理对兔脊髓缺血再灌注损伤细胞内Ca^(2+)变化的影响  

Effect of preconditioning with electroacupuncture on changes of intracellular Ca^(2+)in spinal ischemia-reperfusion injury in rabbits

作　　者：雷毅[1] 熊利泽[1] 张龙芳[2] 曾毅[1] 陈绍洋[1] 黄怡[1] 贺大银[1] 

机构地区：[1]第四军医大学西京医院麻醉科,西安710032 [2]第四军医大学唐都医院耳鼻咽喉科

出　　处：《中国针灸》2004年第4期279-282,共4页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目 ( 3 0 170 90 7)

摘　　要：目的 :探讨重复电针预处理诱导缺血耐受对脊髓缺血再灌注损伤保护作用的机制。方法 :76只雄性新西兰大白兔随机分成 4组 :对照组 (C组 ) ,戊巴比妥钠组 (SP组 ) ,假手术组 (S组 ) ,电针预处理组 (EA组 )。最后一次预处理后 2 4h ,阻闭肾下腹主动脉 ,制作兔脊髓缺血模型 ;S组动物手术操作同C组 ,但不阻闭腹主动脉。分别于阻闭后不同时相点测定细胞内Ca2 + 含量 ,并分别对动物后肢运动功能评分。结果 :EA组脊髓组织细胞内Ca2 + 含量显著低于C组和SP组各时相值 (P <0 0 1) ,神经功能评分EA组明显高于C组 (P <0 0 1) ,C组和SP组细胞内Ca2 + 和神经功能评分无显著性差异 (P >0 0 5 )。结论 :电针预处理具有降低脊髓组织细胞内Ca2 + 含量 ,防止Ca2 + 超载 ,稳定细胞内环境的能力 ,对腹主动脉阻断所致的脊髓缺血再灌注损伤具有良好的保护作用。Objective To study the mechanisms of repeated eletroacupuncture preconditioning inducing ischemic tolerance to spinal ischemia-reperfusion injury. Methods Seventy-six New Zealand white male rabbits were randomly divided into 4 groups: control group (C group), sodium pentobarbital group (SP group), sham operation group (S group) and electroacupuncture preconditioning group (EA group). 24 h after the last preconditioning, spinal cord ischemia was induced by an occlusion of infrarenal aortic artery for 20 min. The treatment in the S group was the same as the C group, but with no the occlusion of the abdominal aortic artery. The levels of intracellular Ca 2+in the spinal cord were determined and the motor function of the hind limbs were assessed at different time after occlusion, respectively. Results The Ca 2+level in the spinal cord in the EA group were significantly lower than those in the C group and SP group at various time (P<0.01), and the neurological score in the EA group was significantly higher than those in the control and the SP groups (P<0.01). Conclusion Preconditioning with EA can significantly decrease the degree of Ca 2+ overload and protect the spinal ischemic injury.

关 键 词：电针 预处理 脊髓缺血 再灌注损伤 细胞内钙离子 神经功能 细胞内环境 

分 类 号：R269.512[医药卫生—中西医结合] R245.97[医药卫生—中医外科学]