益气养阴方对K562细胞bcl-2及p53基因表达的影响  

EFFECTS OF YIQI YANGYIN FORMULA ON EXPRESSION OF bcl-2 AND p53 GENE ON CELL LINES K562

作　　者：臧运华[1] 李震[1] 李洁[1] 高向慧[1] 张丹[1] 李军山[1] 王静丽[1] 王华云[1] 

机构地区：[1]山东中医药大学,山东济南250014

出　　处：《山东中医药大学学报》2004年第3期234-235,共2页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省自然科学基金 (Y2 0 0 0Co3 )

摘　　要：目的 :探讨益气养阴方治疗白血病的机制。方法 :用血清药理学法孵育人白血病细胞系K5 6 2细胞 ,用流式细胞仪检测bcl 2的表达 ,ABC免疫组化法检测突变型p5 3表达。结果 :益气养阴方可使K5 6 2细胞bcl 2和突变型 p5 3基因表达减少。结论 :益气养阴方治疗白血病的机制可能与bcl 2和突变型 p5Objective: To explore the treating mechanism of leukemia with Yiqi Yangyin formula. Methods: Cell lines K562 with human leukemia were hatched by means of serological pharmacology. And then,bcl-2 gene and mutant p53 gene expression on cell lines K562 in rats' serum were examined by flow cytometry and ABC immunohistochemic. Results: Yiqi Yangyin formula down-regulated the expression of bcl-2 gene and mutant p53 gene on the cell lines K562. Conclusions: The functional mechanisms of Yiqi Yangyin formula may be related to the decrease of the expression of bcl-2 and mutant p53 gene.

关 键 词：益气养阴方 K562细胞 BCL-2 P53 基因表达 大鼠 

分 类 号：R285.5[医药卫生—中药学]