玉米BADH基因的克隆与序列分析  

Cloning and Sequencing of BADH Gene from Maize(Zea mays)

作　　者：余爱丽[1] 徐景升[1] 周会[1] 张木清[1] 陈如凯[1] 

机构地区：[1]福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福州350002

出　　处：《分子植物育种》2004年第3期365-368,共4页Molecular Plant Breeding

基　　金：supported by the National Foundation Natural Science of China(No.30170590).

摘　　要：Betaine was accumulated as a nontoxic and protective osmolyte in water-dificit and salt-stressed plants.Be-taine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme.The BADH gene of Maizewas cloned by RT-PCR and RACE.The gene is 1 762 bp in length,including an open reading frame of 1 515 bp.Its nucleotide sequence shares 95% with the the partial cDNA of BADH1 from Sorghum bicolor.Its deducedamino acid sequence contains the conserved domain sequence 'VTLELGGKSP' of ALDH,and a tripeptide SKLat its C-terminal,a signal targetting to the microbodies.The phylogenetic tree of 20 BADHs was constructed,which corresponds to the classical botanical division of plant,the BADH of maize is closest to the one of Oryza Sativa.GenBank accession No.AY587278.<正> Betaine was accumulated as a nontoxic and protective osmolyte in water-dificit and salt-stressed plants. Be-taine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme. The BADH gene of Maize was cloned by RT-PCR and RACE. The gene is 1 762 bp in length, including an open reading frame of 1 515 bp. Its nucleotide sequence shares 95% with the the partial cDNA of BADH1 from Sorghum bicolor. Its deduced amino acid sequence contains the conserved domain sequence 'VTLELGGKSP' of ALDH, and a tripeptide SKL at its C-terminal, a signal targetting to the microbodies. The phylogenetic tree of 20 BADHs was constructed , which corresponds to the classical botanical division of plant, the BADH of maize is closest to the one of Oryza Sativa,GenBank accession No. AY587278.

关 键 词：玉米 BADH基因 基因克隆 序列分析 

分 类 号：S513[农业科学—作物学]