脱水素基因转化的矮牵牛对干旱胁迫的反应  

Dehydrin Gene Transformed Petunia Showed Strong Resistance to Drought Stress

作　　者：王君丹 胡鸢雷[1] 魏晓[1] 于鹏之[1] 车代弟[2] 林忠平[1] 

机构地区：[1]北京大学蛋白质工程和植物基因工程国家重点实验室,北京100871 [2]东北农业大学园艺学院,哈尔滨150030

出　　处：《分子植物育种》2004年第3期369-374,共6页Molecular Plant Breeding

摘　　要：将厚叶旋蒴苣苔脱水素(Dehydrin)基因BDN1置于CaMV35S启动子的调控之下,并插入于表达载体pCAMBIA3300中,通过根癌土壤杆菌(Agrobacterium tumefaciens)介导导入矮牵牛(Petunia Hybrida),经PCR及Southem杂交表明BDN1已转入矮牵牛基因组中,Northern实验证明,BDN1基因在矮牵牛中在RNA水平有较强的表达。通过对转BDN1基因矮牵牛的保水力和低温离子渗漏的测定,初步验证转基因矮牵牛对水分胁迫的能力在一定程度上有所增加。Dehydrin-like Gene (BDN1) from Boea crassifolia was placed under the control of CaMV35S promoter and inserted into binary vector pCAMBIA3300 for genetic transformation. The transgenic plants of Petunia were i-dentified by molecular biology procedure and show strong expression of transgene of dehydrin at transcriptional level. The water conservation ability assay and leaf electrolyte leakage determination showed transgenic petunia plants are with strong tolerance to stress condition.

关 键 词：脱水素 基因转化 矮牵牛 干旱胁迫 

分 类 号：Q943[生物学—植物学]