芍药属牡丹组(Paeonia sect.Moutan)种间关系的分子证据:GPAT基因的PCR-RFLP和序列分析  

Molecular evidence for the interspecific relationships in Paeonia sect. Moutan: PCR-RFLP and sequence analysis of glycerol-3-phosphate acyltransferase (GPAT) gene

作　　者：赵宣[1] 周志钦[1] 林启冰[1] 潘开玉[1] 洪德元[1] 

机构地区：[1]中国科学院植物研究所系统与进化植物学重点实验室

出　　处：《植物分类学报》2004年第3期236-244,共9页Acta Phytotaxonomica Sinica

基　　金：中国科学院知识创新工程重要方向项目 (KSCX2_SW_1 0 8);国家自然科学基金重点项目 (3 0 1 3 0 0 3 0 )资助~~

摘　　要：为了探讨芍药属牡丹组Paeoniasect.Moutan的种间关系 ,对采自 1 5个野生居群 ,代表牡丹组全部 8个野生种的 1 5份材料的GPAT基因片段 (外显子 5和 6之间 2kb的内含子 )进行了PCR_RFLP分析 ,并对代表牡丹组全部 8个野生种的 9份材料进行了测序。根据 1 2个限制性内切酶的PCR_RFLP数据 ,使用Network 3 .0计算机程序的RM (reduced_median)法建立了牡丹组种间亲缘关系网络树。同时根据 8个种 9份材料的GPAT基因片段序列 ,利用PAUP 4.0计算机程序建立了牡丹组GPAT基因的最大简约(MP)树和邻接 (NJ)树。结果获得了具有很高自展值支持、分辨良好的牡丹组种间关系 (GPAT基因 )树。最重要的是 ,该基因树所显示的牡丹组种间关系与根据形态学证据提出的牡丹组的种间关系基本吻合 ,并得到其他研究证据的支持。根据这一结果 ,对牡丹组的种间关系进行了详细的讨论。A portion (the big intron between exon 5 and 6, ca. 2 kb) of glycerol-3-phosphate acyltransferase (GPAT) gene of 15 wild tree peony accessions collected from 15 populations, which represent all the eight wild species in sect. Moutan, was analyzed using PCR-RFLP technique, and this portion of nine accessions, which represent also all the eight wild species, was sequenced for a better understanding of the interspecific relationships in this section. A reduced-median (RM) network of sect. Moutan was constructed with Network 3.0 computer program using the PCR-RFLP data obtained from the digestion experiments of 12 selected restriction enzymes. Both maximum parsimony (MP) and neighbor-joining (NJ) trees of sect. Moutan were constructed with PAUP* 4. 0 program using the sequences newly obtained in this study and from GenBank. As a result, a well resolved and highly supported gene tree of sect. Moutan (by bootstrap values) was obtained. The tree is basically in accordance with that constructed from morphological data. The phylogenetic relationships among species in sect. Moutan are discussed in detail.

关 键 词：芍药属 牡丹组 野生居群 种间关系 GPAT基因 PCR-RFLP 序列分析 

分 类 号：Q949[生物学—植物学]