不同生长调节剂对马蹄金愈伤组织诱导的影响  

Effect of different growth regulators on callus induction from explants in Creeping Dichondra

作　　者：田志宏[1] 李小丽[1] 严寒[1] 邱永福[1] 何勇[1] 

机构地区：[1]湖北农学院生命科学学院,湖北荆州434025

出　　处：《广西植物》2004年第3期253-258,共6页Guihaia

基　　金：湖北省重点科技发展计划资助项目 (992P0 6 0 3) ;湖北省教育厅重大资助项目 (99Z0 0 7)

摘　　要：马蹄金是一种优良的地被兼观赏草坪植物。采用正交设计试验法 ,研究了四种不同生长调节剂对马蹄金子叶、叶片、叶柄和下胚轴愈伤组织诱导的影响。结果表明 :生长调节剂是诱导愈伤组织的关键 ,2 ,4 D对愈伤组织诱导具有显著的影响 ,适宜于马蹄金愈伤组织诱导的培养基及生长调节剂为MS +1 .0mg/L 2 ,4 D +0 .2mg/L 6 BA +0 .2mg/LKT +1 .0mg/Lα NAA。Creeping Dichondra (Dichondra repens) may be considered as a kind of excellent turfgrass and ground cover plants. This experiment inspected the effect of four different growth regulators on callus induction of cotyledon, young leaf, petiole and hypocotyl in Creeping Dichondra with the orthogonal design. The results showed that growth regulator was an important factor on induction of callus, and 2,4-D was essential to induction of callus. The optimum medium supplemented with growth regulators for callus induction of Creeping Dichondra was MS+1.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.2 mg/L KT+1.0 mg/L α-NAA.

关 键 词：生长调节剂 马蹄金 愈伤组织 外植体 旋花科 

分 类 号：Q949.777.1[生物学—植物学] Q945